空间微生物发酵反应器设计及工艺优化研究  

Bioreactor Design and Process Optimization of Microbial Fermentation Technology in Space

作　　者：叶健文 逯耀锋 黄悟哲 吴赴清 陈国强[1] YE Jianwen;LU Yaofeng;HUANG Wuzhe;WU Fuqing;CHEN Guoqiang(School of Life Science,Tsinghua University,Beijing 100084,China;China Manned Space Agency,Beijing 100034,China)

机构地区：[1]清华大学生命科学院,北京100084 [2]中国载人航天工程办公室,北京100034

出　　处：《载人航天》2020年第4期492-497,共6页Manned Spaceflight

基　　金：载人航天预先研究项目(0201)。

摘　　要：针对长期载人航天飞行任务中物质资源的可重复利用及原位再生问题,提出了轻型发酵罐的初步设计。结合嗜盐菌无灭菌发酵特点,通过优化工艺进行微生物高密度培养,对聚羟基脂肪酸酯的生产工艺进行了2种优化测试。结果表明:轻型发酵罐与传统发酵罐性能相当,可以实现超100 g/L的生物量积累,且相较于传统发酵罐减重60%以上。基于优化工艺进行废水循环测试,结果表明:循环利用的发酵液上清仍可保证48%的较高PHA产量,实现了水资源的重复利用。研究结果可为未来在空间开展微生物高密度培养提供可行可靠的解决方案。The recycling and in-situ regeneration of resources are important research topics when dealing with the issue of material replenishment during long-term human spaceflight missions.A new prototyping design of light bioreactor based on non-sterile fermentation process was proposed in this paper to optimize the biomanufacturing of polyhydroxyalkanoates(PHA)and two high cell density culturing approaches were tested.The results showed that the fermentative performance of light plastic bioreactor was equivalent to that of the tradition bioreactor,at the same time it realized over 100 g/L biomass accumulation and more than 60%weight reduction.In addition,recycling of fermentative supernatant was carried out to test the water recycling.The results manifested a promising PHA accumulation with the PHA content reaching 48 wt%and the recycling of water resources was realized.The study may provide a feasible solution for high density bacterial cultivation in the future.

关 键 词：空间微生物发酵 轻型生物反应器 嗜盐菌 聚羟基脂肪酸酯 资源循环利用 

分 类 号：V7[航空宇航科学技术] D590[政治法律—政治学]