shRNA对H1N1及H7N9亚型流感病毒抑制作用的研究  

Inhibiting effects of shRNAs on influenza virus subtypes H1N1 and H7N9

作　　者：史亮 尹沈慧 任浩 高荣 马壮 孙文武 曹建平 Shi Liang;Yin Shenhu;Ren Hao;Gao Rong;Ma Zhuang;Sun Wenwu;Cao Jianping(Department of Respiratory and Critical Care Medicine,General Hospital of Northern Theater Command of PLA,Shenyang,Liaoning,110016,China;Department of Navy Medicine,the Naval Medical University,Shanghai,200433,China)

机构地区：[1]北部战区总医院呼吸与危重症医学科,沈阳110016 [2]海军军医大学海军医学系

出　　处：《西南国防医药》2020年第8期701-704,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：解放军"十二五"科研基金资助项目(CSY14C005)。

摘　　要：目的探讨短发卡RNA(shRNA)对细胞内H1N1及H7N9甲型流感病毒(IVA)的抑制作用。方法合成12种针对H1N1亚型流感病毒保守区域的短发卡RNA(shRNA),观察了这些shRNA对不同亚型流感病毒在细胞中复制的影响。结果转染了质粒pLKD-M-121、pLKD-M-935、pLKD-NP-391、pLKD-NP-1291、pLKD-PA-1365和pLKD-PA-1645的MDCK细胞的H1N1病毒载量显著低于H1N1阳性对照组和空质粒组(P<0.05)。转染了质粒pLKD-M-935、pLKD-NP-391、pLKD-PA-1365、pLKD-PA-1645、pLKD-M-121和pLKD-NP-1291的MDCK细胞的H7N9病毒载量显著低于对照组和空质粒组(P<0.05)。结论针对H1N1亚型的流感病毒基质蛋白(M2),核壳体蛋白(NP)和聚合酶A(PA)片段的shRNAs可以抑制H1N1和H7N9亚型流感病毒的复制。Objective To explore the inhibiting effects of short hairpin RNA(shRNAs) on influenza A virus(IAV) subtypes H1N1 and H7N9 in the cells. Methods A total of 12 types of shRNAs targeting the conserved regions of IAV subtype H1N1 were synthesized, and the effects of these shRNAs on the replication of different subtypes of IAV were observed. Results The load of the H1N1 virus in MDCK cells transfected with plasmids pLKD-M-121, pLKD-M-935, pLKD-NP-391, pLKD-NP-1291 pLKD-PA-1365,and pLKD-PA-1645 was significantly lower than that in the H1N1 positive control group and empty plasmid group(P < 0.05). The load of the H7N9 virus in MDCK cells transfected with plasmids pLKD-M-935, pLKD-NP-391, pLKD-PA-1365, pLKD-PA-1645,p LKD-M-121, and pLKD-NP-1291 was notably lower than that in the control group and empty plasmid group(P < 0.05). Conclusion The sh RNAs targeting segments of matrix protein(M2), nucleocapsid protein(NP), and polymerase(PA) of influenza virus subtype H1N1 can inhibit the replication of influenza virus subtypes H1N1 and H7N9.

关 键 词：甲型流感病毒 RNA 干扰 基质蛋白2 核壳体蛋白 聚合酶A 短发卡RNA 

分 类 号：R373.13[医药卫生—病原生物学]