常春藤非光化学淬灭特性研究  

Characterization of Non-photochemical Quenching in Hedera nepalensis

作　　者：刘哲 谭颖 赵伟[1] 马铭宇 王梦欣 张全胜[1] LIU Zhe;TAN Ying;ZHAO Wei;MA Ming-yu;WANG Meng-xin;ZHANG Quan-sheng(School of Ocean,Yantai University,Yantai 264005,China)

机构地区：[1]烟台大学海洋学院,山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2020年第3期298-306,共9页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：国家自然科学基金资助项目(41376154);烟台市科技发展计划资助项目(2019XDH2096).

摘　　要：本研究采用叶绿素荧光技术研究了常春藤非光化学淬灭(non-photochemical quenching,NPQ)特性,分析了NPQ动力学及光保护效率.结果表明,随光强增强,3种叶龄NPQ最大值(NPQmax)、PSII调节性能量耗散的量子产量[Y(NPQ)]及与PSII非调节性能量耗散量子产量[Y(NO)]的比值[Y(NPQ)/Y(NO)]呈增加趋势,并且NPQ光保护组分增幅大于光抑制组分.成熟叶NPQmax、光保护组分的量子产量、Y(NPQ)、Y(NPQ)/Y(NO)显著高于老叶和幼叶.相对于成熟叶,幼叶和老叶光抑制发生早,光保护效率低.DUAL-PAM-100 P515/535模块测量的跨膜质子梯度(ΔpH),高光下3种叶龄构建的ΔpH均高于低光,成熟叶构建ΔpH能力显著高于其它两种叶龄,意味着ΔpH在NPQ中发挥重要作用.上述结果表明,常春藤NPQ效率随光强增强而增加,成熟叶光保护效率最高.此外,常春藤NPQmax及保护NPQ(protective NPQ,pNPQ)均低于阳生植物,且光抑制发生早,意味着常春藤光保护效率低于阳生植物.In order to examine the characterization of non-photochemical quenching(NPQ)in Hedera nepalensis,the kinetics and the protective efficiency of NPQ are investigated via the chlorophyll a fluorescence technique.With the increase of the light intensity,the maximum for NPQ(NPQmax),the quantum yield of regulated non-photochemical energy loss in PSII[Y(NPQ)]and the yield ratio[Y(NPQ)/Y(NO)]significantly increase.Moreover,the protective components of NPQ increase more than the photoinhibition.NPQmax,the yields for protective components of NPQ,Y(NPQ)and Y(NPQ)/Y(NO)increase in the mature leaf(ML)and decrease in the young leaf(YL)compared to the old leaf(OL).In OL and YL,the onset of photoinhibition takes place at a lower light intensity than in the ML.The protective NPQ(pNPQ)is higher in the ML than that in the OL and YL.The build-up of a proton gradient(ΔpH)is investigated in H.nepalensis utilizing the DUAL-PAM-100 equipped with a P515/535 module.Exposure to high light,the ΔpH building capacity increased.Higher level of the ΔpH in the ML than that in to the OL and YL is observed,indicating that the ΔpH plays an essential role in NPQ.In conclusion,the NPQ efficiency of H.nepalensis enhances with the increasing light intensity and is significantly higher in the ML.In contrast with sun plants,H.nepalensis displays lower NPQmax and pNPQ(protective NPQ),and is more prone to photoinhibition,which means that the photoprotective efficiency is lower in H.nepalensis than in sun plants.

关 键 词：常春藤 非光化学淬灭 叶绿素荧光 跨膜质子梯度 阳生植物 

分 类 号：Q945.79[生物学—植物学]