营养缺陷型大肠杆菌高产L-苏氨酸的研究  

Study on High Production of L-Threonine by Nutritional Deficient Escherichia coli

作　　者：周泉钱 朱长俊[1] 杜雨桐 罗奋杰 钱广[1] Zhou Quan-qian;Zhu Chang-jun;Du Yu-tong;Luo Fen-jie;Qian guang(College of Biological and Chemical Engineering,Jiaxing University.Zhejiang Jiaxing 314001)

机构地区：[1]嘉兴学院生物与化学工程学院,浙江嘉兴314001

出　　处：《生物化工》2020年第4期52-55,共4页Biological Chemical Engineering

基　　金：嘉兴学院大学生创新项目(8517193170)。

摘　　要：对大肠杆菌进行化学诱变获得赖氨酸缺陷型菌株(Lys菌株),在此基础上进一步诱变并筛选出甲硫氨酸缺陷型菌株(Met菌株),最终获得双重缺陷型菌株(Met-Lys菌株),从而提高L-苏氨酸的产量;通过L-苏氨酸和四氯苯醌的荷移反应,可测定普通大肠杆菌、Lys型大肠杆菌和Met-Lys型大肠杆菌发酵后发酵液中L-苏氨酸的含量。结果表明:Lys型大肠杆菌的L-苏氨酸产量比普通型大肠杆菌增加了26.01%,达到了4.07 g/L;而Met-Lys型大肠杆菌的L-苏氨酸产量比普通大肠杆菌增加了299.38%,达到了12.90 g/L。Through chemical mutagenesis of Escherichia coli,the Lysine defective strain(Lys strain)was obtained after screening.On this basis,further mutagenesis and screening of methionine defective strain(Met strain)were carried out,and finally the double defective strain(Met-Lys strain)was obtained,so as to improve the yield of L-threonine.Through the charge transfer reaction of L-threonine and tetrachlorobenzoquinone,the content of L-threonine in the fermentation broth of common Escherichia coli,Lysine-deficient Escherichia coli,Lysine and Methionine-deficient Escherichia coli could be determined.The results showed that the L-threonine production of Lysine-deficient Escherichia coli increased by 26.01% to 4.07 g/L compared with that of normal Escherichia coli.The L-threonine production of Lysine and Methionine double defective Escherichia coli increased by 299.38% compared with that of ordinary Escherichia coli,reaching 12.90 g/L.

关 键 词：化学诱变 营养缺陷型细菌 荷移反应 L-苏氨酸 

分 类 号：R378.21[医药卫生—病原生物学]