稀有细胞群单细胞分选方案的优化  

Optimization of Single Cell Sorting in Rare Cell Groups

作　　者：宋兴辉[1] 李艳伟[1] 王佳佳[1] 邢月婷 黄莹莹[1] 郭春 Song Xing-hui;Li Yan-wei;Wang Jia-jia;Xing Yue-ting;Huang Ying-ying;Guo Chun(Zhejiang University,Zhejiang Hangzhou,310058)

机构地区：[1]浙江大学,浙江杭州310058

出　　处：《生物化工》2020年第4期92-93,96,共3页Biological Chemical Engineering

基　　金：浙江大学实验技术研究项目(SJS201712,SJS201714);浙江省基础公益研究计划项目(LGF19H150006);浙江省教育厅一般科研项目(Y201941524)。

摘　　要：目的:探讨稀有细胞群单细胞分选方案的优化。方法:分析仪器设备的设置、分选前细胞的准备和分选方案的选择。结果:通过提供的单细胞分选的方案,使单克隆形成率达86.5%。结论:建立了单细胞分选的优化方案,并重点分析了提高单克隆形成率的影响因素和解决方法。Objective:To explore the optimization of single cell sorting in rare cell populations.Methods:The setup of instrument and equipment,the preparation of cells before sorting and the selection of sorting scheme were analyzed.Results:Using the single cell sorting scheme provided in this paper,the monoclonal formation rate reached 86.5%.Conclusion:In this paper,the optimization scheme of single cell sorting was established,and the influencing factors and solutions to improve the monoclonal formation rate are analyzed emphatically.

关 键 词：单细胞分选 单克隆形成率 细胞活性 

分 类 号：Q2-33[生物学—细胞生物学]