脓毒症小鼠脾脏CD4+T细胞miR-142-3p的表达与其意义  

Expression and clinical significance of miR-142-3p in CD4+T cells in the spleen of mice with sepsis

作　　者：陈海鸣[1] 韦华璋 朱建光[3] 熊启安 邓陶 温志超 王联群[5] CHEN Haiming;WEI Huazhang;ZHU Jianguang;无(Department of Emergency Surgery,The First Affiliated Hospital of Nanchang University,Nanchang 330006,China;Department of critical care medicine,The Affiliated Hospital of Jinggangshan University,Ji-an 34300,China;Department of critical care medicine,Anfu People's Hospital,Anfu 34300,China;The Fist Clinical Medical College of Nanchang University,Nanchang 330006;Department of critical care medicine,The First Affiliated Hospital of Nanchang University,Nanchang 330006,China)

机构地区：[1]南昌大学第一附属医院急诊外科,南昌330006 [2]井冈山大学附属医院重症医学科,吉安34300 [3]安福县人民医院重症医学科,安福34300 [4]南昌大学第一临床医学院,南昌330006 [5]南昌大学第一附属医院重症医学科,南昌330006

出　　处：《江西医药》2020年第8期1021-1023,共3页Jiangxi Medical Journal

基　　金：江西省自然科学基金,编号20171BAB205030;江西省教育厅重点项目,编号GJJ160040。

摘　　要：目的观察脓毒症小鼠脾脏CD4+T细胞的miR-142-3p的表达水平。方法通过盲肠结扎穿孔法建立脓毒症小鼠模型,28只C57BL/6小鼠随机(随机数字法)分为两组:假手术组,脓毒症模型组,每组各14只。所有小鼠均于手术后48 h处死并取材。采用酶联免疫吸附法检测小鼠血清IL-1β、TNF-α、IL-6、IL-2,通过实时荧光定量PCR(qRT-PCR)方法检测小鼠脾脏CD4+T细胞miR-142-3p的水平。结果脓毒症小鼠脾脏CD4+T细胞的miR-142-3p表达水平较假手术组显著升高(P<0.05),IL-1β、TNF-α、IL-6、IL-2水平均较假手术组显著升高(P<0.05)。结论脓毒症小鼠脾脏CD4+T细胞中miR-142-3p表达上调与炎症相关。Objective To observe the expression level of miR-142-3p in CD4+T cells in the spleen of mice with sepsis.Methods Mice with sepsis was induced by means of cecal ligation and puncture(CLP),28 mice on a C57BL/6 background were randomly divided into two groups:14 in the sham surgery group and 14 in the sepsis model group.All mice were sacrificed at 24 h post-surgery and samples were collected.The serum samples were used to measure the level of IL-1β、TNF-α、IL-6、IL-2 by ELISA,and the expression level of miR-142-3p in CD4+T cell in the spleen was measured by qRT-PCR(Quantitative real time polymerase chain reaction).Results In the sepsis model group,the expression level of miR-142-3p in CD4+T cell in the spleen was increased dramatically compared to those of the sham surgery group(P<0.05),as well as the level of IL-1β、TNF-α、IL-6、IL-2 in the serum samples(P<0.05).Conclusion miR-142-3p in CD4+T cell in the spleen of mice with early sepsis were up-regulated.

关 键 词：微小RNA-142-3p 脓毒症 CD4+T细胞 细胞因子 

分 类 号：R459.7[医药卫生—急诊医学]