不同培养基对云烟97变异株腋芽培养的效应研究  

Effects of Different Media on Culture of Axillary Buds of Yunyan 97 Mutant

作　　者：刘瑞婕 张丽琼 李炳 陈佳荷 张子怡 LIU Ruijie;ZHANG Liqiong;LI Bing;CHEN Jiahe;ZHANG Ziyi(School of Modern Agriculture and Biotechnology,Ankang University,Ankang,Shaanxi 725000,China;Research Center for Rural Revitalization in Southern Shaanxi,Ankang 725000,China)

机构地区：[1]安康学院现代农业与生物科技学院,陕西安康725000 [2]陕南乡村振兴研究中心,陕西安康725000

出　　处：《农业研究与应用》2020年第3期19-24,共6页Agricultural Research and Application

基　　金：陕西省大学生创新创业训练计划项目(2017sxjy002)。

摘　　要：以云烟97变异株腋芽为外植体,研究不同培养基对云烟97变异株组织培养的效应。结果表明,初代培养基MS+6-BA 2.0 mg/L分化成芽率高达75%;初代培养基MS+6-BA 1.0 mg/L,继代培养基MS+6-BA 1.0mg/L+NAA 0.2mg/L分化成芽率高达77%;初代培养基MS+6-BA 2.0 mg/L,继代培养基MS+6-BA 3.0 mg/L分化成芽率高达76%。故利用植物快繁技术培养云烟97时,要根据初代培养基选择相应的继代培养基。Axillary buds of Yunyan 97 mutant were used as explants to study the effects of different media on axillary buds formation of Yunyan 97 mutant.Results showed that the bud formation rate in the initial medium:MS+6-BA 2.0 mg/L was up to 75%;in the initial medium:MS+6-BA 1.0 mg/L and the subculture medium:MS+6-BA 1.0 mg/L+NAA 0.2 mg/L reached 77%;in the initial medium:MS+6-BA 2.0 mg/L and subculture medium:MS+6-BA 3.0 mg/L reached76%.It is suggested to select corresponding subculture medium based on initial medium to culture Yunyan 97 by rapid propagation technology.

关 键 词：烟草 云烟97 变异株 腋芽 组织培养 

分 类 号：S572[农业科学—烟草工业]