南昌市2014—2017年活禽市场禽流感病毒监测  

Surveillance on the avian influenza virus in live poultry markets in Nanchang city from 2014 to 2017

作　　者：冯道斌 宋文涛[2] 倪贤生 吴景文[2] 王艳晴 陈盛恩[2] 陈海婴[2] 刘明斌 Feng Daobin;Song Wentao;Ni Xiansheng;Wu Jingwen;Wang Yanqing;Chen Sheng'en;Chen Haiying;Liu Mingbin(School of Public Health,Nanchang University,Jiangxi Provincial Key Laboratory of Preventive Medicine,Nanchang 330006,China;Nanchang Center for Disease Control and Prevention,Nanchang 330038,China;Shanghai Public Health Clinical Center Affiliated to Fudan University,Shanghai 201508,China)

机构地区：[1]南昌大学公共卫生学院,江西省预防医学重点实验室,330006 [2]南昌市疾病预防控制中心,330038 [3]复旦大学附属公共卫生临床中心,上海201508

出　　处：《中华实验和临床病毒学杂志》2020年第3期264-269,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金项目(81660339)。

摘　　要：目的了解南昌市活禽市场中禽流感病毒的分布状况,为评估新型禽流感病毒(avian influenza virus,AIV)的潜在公共卫生威胁及其预防控制提供科学依据。方法采用整群随机抽样的方法抽取南昌市10个县/区47家活禽市场作为监测场所。采用Real time RT-PCR对采集的活禽和环境标本进行AIV核酸检测。使用Excel 2016、SPSS 24.0和Prism Version 7(GraphPad)软件进行统计学分析。结果2014—2017年共采集活禽拭子和市场环境标本9248份,AIV核酸阳性标本1525份,总体阳性率为16.49%。H7、H9是活禽市场的主要流行亚型,H9亚型阳性率最高(17.44%)。清洗禽类污水(21.46%)和禽咽拭子(20.46%)的阳性率显著高于其他类型标本。鸡(22.80%)和鸭(13.95%)的阳性率在所有家禽中排前2位。红谷滩新区(25.52%)、安义县(24.31%)、新建区(22.35%)的阳性率高于其他县区,不同县/区AIV亚型构成不同。结论南昌市活禽市场中H7、H9等多种亚型AIV共存循环,应采取持续的主动监测和综合控制措施以降低人群潜在的感染风险。To investigate epidemic status of avian influenza virus(AIV)in live poultry market(LPM)and evaluate its potential public health threat and develop control measures in Nanchang city.Methods Forty-seven LPM in 10 counties/districts of Nanchang city were selected as surveillance sites by using cluster random sampling.Poultry swabs and environmental samples were obtained from LPM.Real time RT-PCR was used for AIV typing and subtyping.Excel 2016,SPSS 24.0 and Prism Version 7(GraphPad)software were used for statistical analysis.Results Total of 9248 poultry swabs and environmental samples were collected.1525 specimens(16.49%)were positive for AIV.H7 and H9 were the dominant subtypes,and H9 positive rate was highest(17.44%).Sewage(21.46%)and throat swabs(20.46%)had higher positive rates than the other types of samples.Chicken and duck were dominant hosts for AIV,the corresponding positive rates were 22.80%and 13.95%.The positive rates of Honggutan district,Anyi county and Xinjian district were 25.52%,24.31%and 22.35%,which were higher than that of the other counties/districts.Each county/district had its unique subtype composition of AIV.Conclusions The coexistence of H7,H9 and other subtypes of AIV in LMP was identified in Nanchang city.Continuing active surveillance and comprehensive control measures should be taken to reduce the potential risk of human infection.

关 键 词：禽 环境 禽流感病毒 核酸 亚型 

分 类 号：R511.7[医药卫生—内科学] R181.8[医药卫生—临床医学]