甘肃省2010—2018年度流感病毒监测结果分析  

Analysis on the surveillance results of influenza virus in Gansu province, 2010-2018

作　　者：张慧[1] 李红育[1] 徐丛杉 李保娣[1] 姜中毅[1] 于德山[1] 刘子梅 何健[1] Zhang Hui;Li Hongyu;Xu Congshan;Li Baodi;Jiang Zhongyi;Yu Deshan;Liu Zimei;He Jian(Gansu Center for Disease Control and Prevention,Lanzhou 730000,China;Gansu Medical College,Pingliang 744000,China)

机构地区：[1]甘肃省疾病预防控制中心,兰州730000 [2]甘肃省医学院,平凉744000

出　　处：《中华实验和临床病毒学杂志》2020年第3期276-279,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家十三五重大专项(20172X10103006001,20172X10104001)。

摘　　要：目的分析甘肃省2010—2018年度流感监测结果及流行特征,为流感防控策略的制定和调整提供依据。方法在甘肃省19家国家级流感监测哨点医院采集流感样病例(influenza-like illness,ILI)标本,全省各级流感网络实验室采用Real-time RT-PCR法进行核酸检测,用犬肾细胞(canine kidney cell,MDCK)进行流感病毒分离培养。结果2010年1月份至2018年12月份共检测ILI标本93334份,检出流感病毒核酸阳性标本13440份,流感病毒核酸检测阳性率为14.40%,阳性标本中乙型流感病毒所占比率最高(37.02%),其次为季节性H3N2型流感病毒(35.25%)、甲型H1N1型流感病毒(26.78%)。不同时期优势型别不同。每年12月份至次年3月份为流感高峰期,其中5~14岁年龄组发病率最高(20.34%),其余年龄组发病率相近,不同年龄组流感阳性率差异有统计学意义(χ2=775.153,P<0.001)。男性、女性流感病毒感染无统计学差异。对16101份ILI标本进行病毒分离培养,共分离到3892株流感病毒,其中乙型流感病毒占比最高(37.82%),其次为季节性H3N2型流感病毒(33.04%)、甲型H1N1型流感病毒(28.96%)。结论甘肃省流感病毒的流行规律较为明显,呈冬春季单峰流行,要加强对学龄期青少年儿童的流感防控。Objective To analyze the influenza surveillance results and epidemiological characteristics of the disease in Gansu province in 2010-2018,and provide scientific basis for the formulation and adjustment of influenza prevention and control strategies.Methods Real-time PCR was used to detect the specimens of ILI illness(ILI)cases reported by 19 national influenza surveillance sentinel hospitals in Gansu province.Influenza virus isolation and culture were carried out using canine kidney cells(MDCK).Results Total of 93334 specimens from ILI cases were collected from Jan.2010 to Dec.2018,and 13439 samples were positive for influenza virus nucleic acid.The positive rate of viral nucleic acid detection was 14.40%,and the proportion of influenza B virus in positive samples was the highest(37.02%),and seasonal H3N2 influenza virus(35.25%),H1N1 influenza virus(26.78%).The advantages are different in various periods.The peak period of influenza is from Dec.to Mar.,and the highest incidence rate is in the age group of 5-15 years(20.34%).The incidence rates of the other age groups are similar.The difference of influenza positive rate in different age groups is statistically significant(χ2=775.153,P<0.001),but in male and female was not significant.Total of 16101 ILI specimens positive for influenza virus nucleic acid were subjected to virus isolation and cultured,and 3892 strains of influenza virus were isolated,of which influenza B virus accounted for the highest proportion(37.82%),followed by seasonal H3N2 influenza virus(33.04%),H1N1 influenza virus(28.96%).Conclusions The epidemic of influenza in Gansu province had an obvious regularity,and it was undulating and prevalent.It is necessary to strengthen prevention and control measures among school-age children and adolescents.

关 键 词：流感样病例 流感病毒 监测 核酸检测 病毒分离 

分 类 号：R181.8[医药卫生—流行病学] R511.7[医药卫生—公共卫生与预防医学]