福州市腹泻住院儿童中A组双埃可病毒流行情况及基因型别分析  

Prevalence and genotypes of parechovirus A in hospitalized children with acute gastroenteritis in Fuzhou, China

作　　者：黄枝妙 吴冰珊[1] 林卫东 翁育伟[1] Huang Zhimiao;Wu Bingshan;Lin Weidong;Weng Yuwei(Fujian Provincial Center for Disease Control and Prevention,Fujian Provincial Key Laboratory of Zoonosis Research,Fuzhou 350001,China;Fuzhou Pediatric Hospital,Fuzhou 350004,China)

机构地区：[1]福建省疾病预防控制中心,福建省人兽共患病研究重点实验室,福州350001 [2]福州市儿童医院,350004

出　　处：《中华实验和临床病毒学杂志》2020年第3期289-292,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家科技重大专项(2017ZX10104001)。

摘　　要：目的探讨福州市5岁以下腹泻住院儿童中A组双埃可病毒(parechovirus A,PeV-A)的流行情况和基因型别分布。方法通过Real-time RT-PCR方法对2018年福州市腹泻监测哨点医院5岁以下腹泻住院儿童粪便标本进行PeV-A筛查;采用巢式RT-PCR方法扩增PeV-A阳性标本的VP3/VP1连接片段并进行测序分型。结果在316例标本中检测出PeV-A阳性标本28例,阳性率为8.86%,其中2例混合杯状病毒感染,与轮状病毒、腺病毒和星状病毒无混合感染。阳性标本中成功分型24例,包括21例PeV-A1、1例PeV-A3和2例PeV-A6,主要流行型别为PeV-A1型,占87.5%(21/24)。PeV-A感染主要发生在1岁以下婴幼儿群体中,感染高峰期在8~10月份。结论PeV-A1是引起福州腹泻住院儿童的主要病原体,主要以1岁以下婴幼儿为感染对象。Objective To investigate the prevalence and genotypes of parechovirus A(PeV-A)in hospitalized children with acute gastroenteritis under 5 years of age in Fuzhou,China.Methods We performed a real-time RT-PCR to screen for PeV-A from stool samples and amplify VP3/VP1 junction region by nested RT-PCR to identify PeV-A type.Results The result showed that 28 of 316(8.86%)children with acute gastroenteritis were positive for PeV-A,two cases were co-infected with calicivirus,none with rotavirus,adenovirus or astrovirus.Totally 21 cases were PeV-A1,1 case was PeV-A3 and 2 cases were PeV-A6 among 24 cases who were positive for PeV-A;PeV-A1 was the most prevalent strain with the proportion of 87.5%.The infection with PeV-A mainly occurred in children under 1 year of age and was prevalent between August and October.Conclusions PeV-A1 was a main pathogen in PeV-A in hospitalized children with acute gastroenteritis in Fuzhou,and the children under 1 year of age were the main target population of PeV-A1.

关 键 词：A组双埃可病毒 基因型别 住院儿童 腹泻 

分 类 号：R725.1[医药卫生—儿科]