加味芍药甘草汤、电针“曲池”“阳陵泉”穴位及针药结合方法对SCA大鼠皮质中γ-氨基丁酸能相关受体的影响  

Effect of Modified Shaoyao Gancaotang,Electroacupuncture and Acupuncture Combined with Herb onγ-Aminobutyric Acid Related Receptor Proteins in Cortex in SCA Rats

作　　者：郭斌 王彭汉 黄麟荇 谢志强 谢丽娜 岳增辉[1] GUO Bin;WANG Peng-han;HUANG Lin-xing;XIE Zhi-qiang;XIE Li-na;YUE Zeng-hui(College of Acupuncture&Moxibustion and Tui-na,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学针灸推拿学院,长沙410208

出　　处：《中国实验方剂学杂志》2020年第17期29-35,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81673886);湖南省自然科学基金项目(2018JJ2294);湖南省中医药科研计划项目(201862)。

摘　　要：目的:分析脑卒中痉挛状态(SCA)大鼠肌张力损伤情况与大脑皮质区γ-氨基丁酸α受体(GABAα),γ-氨基丁酸转运体(GAT-1)蛋白及mRNA表达情况,探讨加味芍药甘草汤、电针曲池、阳陵泉穴位及针药结合3种方法缓解脑卒中肢体痉挛的疗效。方法:通过2次随机分组,分为空白组、假手术组、模型组、加味芍药甘草汤组、针药结合组、电针组,每组9只。采用改良Zea-Longa线栓法+内囊注射NMDA受体法制备脑卒中肢体痉挛大鼠模型,行为学评分确认模型成功后,分别对电针组(电针双侧阳陵泉、曲池,30 min/次/d,持续5 d),加味芍药甘草汤组(加味芍药甘草汤水煎剂1次/d,每次灌胃10 mL·kg-1,持续5 d),针药结合组(加味芍药甘草汤灌服;再行电针治疗,持续5 d)进行干预。治疗后行为学检测的肌张力情况,采用实时荧光定量PCR(Real-time PCR)和蛋白免疫印迹法(Western blot)检测皮质中GABAα,GAT-1 mRNA与蛋白的表达。结果:与正常组比较,模型组肌张力显著增高(P<0.01),GABAαmRNA与蛋白的表达显著降低(P<0.01),GAT-1 mRNA与蛋白的表达显著上升(P<0.01);与模型组比较,各治疗组肌张力评分明显降低(P<0.05),GABAαmRNA与蛋白的表达明显升高(P<0.05,P<0.01),GAT-1 mRNA与蛋白的表达明显下降(P<0.05,P<0.01),其中针药结合组相应表达最显著。结论:加味芍药甘草汤、电针曲池、阳陵泉穴位及针药结合三种疗法均可以缓解脑卒中肢体痉挛的肌张力情况,而针药结合治疗方法对皮质中γ-氨基丁酸能受体表达情况效能最高,提示其改善脑卒中肢体兴奋性痉挛状态的作用最优。Object:To analyze the effect of muscular tension injury in spasticity of cerebral apoplexy(SCA)rats with spasticity and the expression ofγ-aminobutyric acid receptor(GABAα),γ-aminobutyric acid transporter(GAT-1)protein and mRNA expression in cerebral cortex modified Shaoyao Gancaotang,electroacupuncture Quchi,Yanglingquan and acupuncture combined with herb therapies alleviate the effect of SCA.Method:Randomly divided into two groups,divided into blank group,sham operation group,model group,modified Shaoyao Gancaotang group,acupuncture combined with herb group,electroacupuncture group,selected qualified SD rats into the group,9 rats in each group.The modified Zea-Longa thread embolism method+internal capsule injection of NMDA receptor method was used to prepare a rat model.After the behavioral score confirms the success of the model,dispose of each group separately,electroacupuncture group(electroacupuncture on both sides of Yangling Spring and Quchi,1 time/d for 5 days),modified Shaoyao Gancaotang group(with Shaoyao Gancaotang 1 time/d,10 mL·kg-1 for 5 days),acupuncture combined with herb(modified Shaoyao Gancaotang was given,then electroacupuncture treatment was continued for 5 days).After treatment,the muscle tension was detected by behavioral analysis.Real-time fluorescent quantitative PCR(Realtime PCR)and Western blot were used to detect the expression of GABAαand GAT-1 related mRNA and protein in the cortex.Result:Compared with normal group,the muscle tension of the model group increased(P<0.01),the expression of GABAαmRNA and protein decreased(P<0.01),and the expression of GAT-1 mRNA and protein increased(P<0.01),compared with model group,the muscle tone score of the each treatment group decreased(P<0.05),the expression of GABAαmRNA and protein increased(P<0.05,P<0.01),and the expression of GAT-1 mRNA and protein decreased(P<0.05,P<0.01),of which the corresponding expression in the acupuncture combined with herb group was the most significant.Conclusion:Electroacupuncture Quchi,Yanglingquan,modified

关 键 词：脑卒中痉挛状态 γ-氨基丁酸α受体 γ-氨基丁酸转运体1 电针 芍药甘草汤 针药结合 

分 类 号：R2-0[医药卫生—中医学] R289