刀豆蛋白A诱导的小鼠自身免疫性肝炎T细胞亚群特点分析  

Characteristics of T cell subsets on Concanavalin A induced autoimmune hepatitis in mice

作　　者：刘录录 孙朋 李秋田 王圣刚[2] 张国友[2] LIU Lulu;SUN Peng;LI Qiutian;WANG Shenggang;ZHANG Guoyou(Department of Surgery,Weifang Medical University,Weifang 261053,China;Department of Gastrointestinal Anus Surgery,the Affiliated Hospital of Weifang Medical University)

机构地区：[1]潍坊医学院外科学教研室,山东濰坊261053 [2]潍坊医学院附属医院胃肠肛门疾病外科

出　　处：《潍坊医学院学报》2020年第3期234-236,共3页Acta Academiae Medicinae Weifang

摘　　要：目的研究尾静脉注射刀豆蛋白A(ConA)24h后小鼠脾脏CD4^+T,CD8^+T细胞的比例及CD4^+T/CD8^+T的变化情况,进一步研究CD4^+CD25^+Treg和CD8^+CD25^+Treg的表达特点,从而探讨ConA诱导自身免疫性肝炎(AIH)模型中T细胞的表型特点,为研究自身免疫性肝炎的发病机制提供依据。方法 8周龄雌性C57BL/6小鼠,平均体重20g。随机分为正常对照组、ConA造模组,每组10只,分别尾静脉注射PBS、ConA(浓度15mg/kg),24h后流式检测小鼠脾脏CD4^+T,CD8^+T,CD4^+/CD8^+,CD4^+Treg,CD8^+Treg的变化情况。结果注射ConA小鼠脾脏T细胞表达增多,以CD4^+T细胞浸润为主,其中CD4^+Treg,CD8^+Treg比例明显降低。结论 ConA诱导的小鼠自身免疫性肝炎中CD4^+T的亚群平衡出现紊乱,可以模拟AIH的发病特点,为AIH的深入研究奠定基础。Objective To study the ratio of CD4^+T,CD8^+T cells and the expression of CD4^+T/CD8^+T in the spleen of mice 24 hours after concanavalin A(ConA) injected into the tail vein,and to further study the expression characteristics of CD4^+,CD25^+Treg and CD8^+,CD25^+Treg,so as to explore the phenotype characteristics of T cells in the model of autoimmune hepatitis induced by ConA,and to provide the basis for further study of the pathogenesis of autoimmune hepatitis.Methods The 8-week-old female C57 BL/6 mice with an average weight of 20 g were randomly divided into two groups including control group,ConA model group,10 mice in each group,respectively injected with PBS and ConA(concentration 15 mg/kg) via tail vein.Splenocytes were harvested 24 h after ConA injection for the detection of the percentages of splenic CD4^+T,CD8^+T,CD4^+/CD8^+and CD4^+Treg,CD8^+Treg by using flow cytometry.Results ConA increased the expression of T cells in the spleen of mice,which was mainly infiltrated by CD4^+T cells,the proportion of CD4^+Treg and CD8^+Treg decreased significantly.Conclusion ConA mice injected with spleen T cells increased expression,mainly by CD4^+ T cell infiltration,of which CD4^+Treg,CD8^+Treg ratio decreased significantly.

关 键 词：刀豆蛋白A 自身免疫性肝炎 T淋巴细胞 调节性T细胞 

分 类 号：R593.2[医药卫生—内科学]