化脓隐秘杆菌HJ-1型生长曲线与感染小鼠半数致死量的测定  

Determination of Growth Curve and LD-50 of Trueperella Pyogenes Type HJ-1

作　　者：赵明迪 范春玲[1] 周玉龙[1] 孙斌[1] 郭东华[1] 原冬伟 吴海燕[1] 崔超伟 燕传利 Zhao Mingdi;Fan Chunling;Zhou Yulong;Sun Bin;Guo Donghua;Yuan Dongwei;Wu Haiyan;Chui Chaowei;Yan Chuanli(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319;Heilongjiang Province Qinglongshan State Farm;Animal Husbandry Bureau of Guangrao,Shandong Province)

机构地区：[1]黑龙江八一农垦大学,大庆163319 [2]黑龙江省青龙山农场 [3]山东省广饶县畜牧局

出　　处：《黑龙江八一农垦大学学报》2020年第4期20-24,52,共6页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省农垦总局重点科研计划项目(HKKYZD190312)。

摘　　要：为了研究化脓隐秘杆菌的危害性,实验测定了化脓隐秘杆菌HJ-1型的细菌生长曲线和其对小鼠的半数致死量。首先对化脓隐秘杆菌HJ-1型菌株进行分离培养,使用分光光度计对菌液进行实时OD600值的测定,同时对各时间段的菌液进行细菌计数,绘制出化脓隐秘杆菌HJ-1型的细菌生长曲线;根据改进寇氏算法计算出化脓隐秘杆菌HJ-1感染小鼠的半数致死量。结果表明,化脓隐秘杆菌HJ-1型在5%血清的BHI液体培养基中,培养12~14 h为该菌的对数生长期,此时的细菌数为8.4×1010cfu;化脓隐秘杆菌HJ-1型对小鼠的半数致死量LD50=4.16×108cfu。Growth curve and LD-50 of Trueperella pyogenes(T.Pyogenes)type HJ-1 was determined to study on the harmfulness of T.Pyogenes.Firstly,T.Pyogenes type HJ-1 was isolated and cultured.The real-time OD600 value of the bacterial solution was determined by spectrophotometer.At the same time,the bacterial solution of each time period was counted.Then the bacterial growth curve of T.Pyogenes type HJ-1 was plotted.LD-50 of T.Pyogenes type HJ-1 was determined by the improved Karber method.The results showed that the logarithmic growth period of T.Pyogenes type HJ-1 was 12-14 h in BHI liquid medium with 5%serum,the bacterial count at this time was 8.4×10^10 cfu and LD-50 was 4.16×10^8 cfu of T.Pyogenes type HJ-1 to mice.

关 键 词：化脓隐秘杆菌HJ-1 生长曲线 半数致死量 

分 类 号：Q78[生物学—分子生物学]