不同来源大果木姜子挥发油及脂肪油成分的比较分析  

Comparative Analysis of Volatile Oil and Fatty Oil Constituents from Cinnamomum migao in Different Sources

作　　者：黄凯 刘杰 黄彩河 刘耀 程纯 张永萍 徐剑 HUANG Kai;LIU Jie;HUANG Caihe;LIU Yao;CHENG Chun;ZHANG Yongping;XU Jian(School of Pharmacy,Guizhou University of TCM,Guiyang 550025,China;Guizhou Yibai Women’s Pharmaceutical Co.,Ltd.,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,贵阳550025 [2]贵州益佰女子大药厂有限责任公司,贵阳550025

出　　处：《中国药房》2020年第16期1961-1966,共6页China Pharmacy

基　　金：国家自然科学基金委员会-贵州省人民政府联合基金项目(No.U1812403-2);贵阳中医学院2017年度学术新苗培养及创新探索专项项目(No.黔科合平台人才〔2017〕5735号-07);贵州省高层次创新型人才“百层次人才”项目(No.黔科合平台人才〔2015〕4030号)。

摘　　要：目的:探讨不同来源大果木姜子挥发油及脂肪油成分的差异。方法:采用水蒸气蒸馏法和索氏提取法分别提取不同来源大果木姜子挥发油和脂肪油,并计算各自的提取率;采用气质联用法分析不同来源大果木姜子挥发油和脂肪油中的化学成分;通过NIST 17、WILEY 275数据库和质谱计算机数据系统进行化合物检索、匹配,采用峰面积归一化法计算各化合物的相对百分含量。结果:4批不同来源大果木姜子挥发油的提取率分别为3.1%、4.5%、6.2%、5.5%,脂肪油的提取率分别为6.2%、8.3%、10.5%、9.4%。从不同来源大果木姜子挥发油中共鉴定出87个成分,其中S1中共分离出104个成分,鉴定出67个,相对百分含量为90.172%;S2中共分离出102个成分,鉴定出73个,相对百分含量为88.836%;S3中共分离出77个成分,鉴定出57个,相对百分含量为93.972%;S4中共分离出87个成分,鉴定出60个,相对百分含量为95.247%;这87个成分中,单萜类及其衍生物型挥发性成分有48个、倍半萜类及其衍生物型挥发性成分33个、脂肪族类挥发性成分4个、酮类挥发性成分2个。不同来源大果木姜子挥发油有44个共有成分,均为萜类成分,在S1~S4中的相对百分含量分别为38.556%、66.776%、88.886%、90.115%;44个共有成分中相对百分含量均大于1%的成分分别为1,8-桉叶素(S2:6.518%;S4:3.850%;S3:1.655%;S1:1.475%)、4-松油醇(S2:1.591%;S4:1.384%;S3:1.193%;S1:1.182%)、α-萜品烯醇(S3:8.662%;S4:7.173%;S2:6.503%;S1:4.839%)、δ-杜松烯(S3:8.597%;S4:5.329%;S2:2.677%;S1:2.547%)、榄香醇(S3:4.781%;S2:4.113%;S1:2.568%;S4:1.897%)和γ-桉叶油醇(S2:4.061%;S3:2.167%;S1:1.575%;S4:1.197%)。从不同来源大果木姜子脂肪油中共鉴定出37个成分,其中S1中共分离出87个成分,鉴定出34个,相对百分含量为91.072%;S2中共分离出69个成分、鉴定出28个,相对百分含量为90.527%;S3中共分离出63个成分,鉴定出23个,相对百分含量为85.297%;S4中共分离OBJECTIVE:To compare the difference of volatile oil and fatty oil constituents from Cinnamomum migao in different sources.METHODS:The steam distillation method and Soxhlet extraction mothod were used to extract volatile oil and fatty oil from C.migao in different sources respectively,and the extraction rates were calculated;GC-MS was used to analyze volatile oils and fatty oils constituents from C.migao in different sources.The compounds were searched and matched through NIST 17,WILEY 275 databases and mass spectrometry computer date system.The relative percentage content of each constituent was calculated by peak area normalization method.RESULTS:The extraction rates of the volatile oils from 4 batches of C.migao in different sources were 3.1%,4.5%,6.2%and 5.5%,respectively;the extraction rates of the fatty oils from C.migao were6.2%,8.3%,10.5%and 9.4%,respectively.A total of 87 constituents were identified in 4 batches of volatile oils of C.migao in different sources,of which 104 constituents were separated from S1,67 were identified,and the relative percentage content was 90.172%;102 constituents were separated from S2,73 were identified,and the relative percentage content was 88.836%;77 constituents were separated from S3,57 were identified,with a relative percentage content of93.972%;87 constituents were separated from S4,60 were identified,with a relative percentage content of 95.247%.Among above 87 constituents,48 were monotyloids and their derivatives,33 were sesquiterpenoids and their derivatives,4 were aliphatic and 2 were ketones.There were 44 common constituents from the volatile oil of C.migao in different sources,all of which were terpenoids.The relative percentage content of S1-S4 were 38.556%,66.776%,88.886%and 90.115%,respectively.Among 44 common constituents,the relative percentage content of which were all greater than 1%were 1,8-cineole(S2:6.518%;S4:3.850%;S3:1.655%;S1:1.475%;),4-terpineol(S2:1.591%;S4:1.384%;S3:1.193%;S1:1.182%),α-terpinenol(S3:8.662%;S4:7.173%;S2:6.503%;S1:4.839%),δ-cadine

关 键 词：大果木姜子 来源 气质联用法 挥发油 脂肪油 成分比较 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]