EoNPV对茶尺蠖两近缘种的毒力差异  

Toxicity difference of EoNPV of two sibling species of tea genometrids

作　　者：李红 唐美君[1,3] 郭华伟 王志博[1,3] 肖强 LI Hong;TANG Meijun;GUO Huawei;WANG Zhibo;XIAO Qiang(Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;Graduate School of the Chinese Academy of Agricultural Sciences, Beijing 100081, China;Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China)

机构地区：[1]中国农业科学院茶叶研究所,浙江杭州310008 [2]中国农业科学院研究生院,北京100081 [3]农业农村部茶叶质量安全控制重点实验室,浙江杭州310008

出　　处：《浙江农业学报》2020年第8期1415-1419,共5页Acta Agriculturae Zhejiangensis

基　　金：国家重点研发计划(2019YFD1002100);中国农业科学院创新工程(CAAS-ASTIP-2019-TRICAAS)。

摘　　要：茶尺蠖核型多角体病毒(Ectropis obliqua nucleopolyhedrosis virus,EoNPV)是一种重要的病原微生物,为进一步明确该病毒对茶尺蠖两近缘种的毒力差异,采用叶盘法对3龄茶尺蠖和灰茶尺蠖幼虫进行了EoNPV的毒力测定。研究显示,EoNPV对灰茶尺蠖的半致死剂量LD50(median lethal dose)为茶尺蠖的28.9倍,EoNPV对灰茶尺蠖的致死中时间LT50(median lethal time)大于茶尺蠖。结果表明,EoNPV对茶尺蠖两近缘种间的毒力存在差异,EoNPV对茶尺蠖具有更高的致病力,研究结果对EoNPV田间防治茶尺蠖两近缘种具有现实指导意义。The EoNPV is an important pathogenic microorganism.And EoNPV can cause the death of Ectropis obliqua Prout and Ectropis grisescens in tea plantation.In order to further clarify the virulence difference of EoNPV to two sibling species of E.obliqua and E.grisescens.The leaf disk method feeding with two sibling species 3th instar larvae was used to find out the difference in virulence.The results showed that LD50 of E.grisescens was 28.9 times as E.obliqua.The LT50 of E.obliqua was less than E.grisescens.It showed that toxicity was different in two sibling species of tea genometrids.EoNPV had higher pathogenicity to E.obliqua.It had practical significance for using EoNPV to control the two sibling species of tea plantation.

关 键 词：茶尺蠖 灰茶尺蠖 茶尺蠖核型多角体病度 叶盘法 半致死剂量 致死中时间 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]