滇产两面针中抗肿瘤活性生物碱成分研究  

Study on the alkaloids and their anticancer activity from Zanthoxylum nitidum

作　　者：邓颖 沈晓华 邓璐璐 郝小江[2,3] 穆淑珍 DENG Ying;SHEN Xiao-Hua;DENG Lu-Lu;HAO Xiao-Jiang;MU Shu-Zhen(College of Pharmacy,Guizhou University,Guiyang 550025,China;State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University;Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,China)

机构地区：[1]贵州大学药学院,贵阳550025 [2]贵州医科大学省部共建药用植物功效与利用国家重点实验室 [3]贵州省中国科学院天然产物化学重点实验室,贵阳550014

出　　处：《天然产物研究与开发》2020年第8期1370-1378,共9页Natural Product Research and Development

基　　金：国家自然科学基金(31760097)。

摘　　要：从滇产两面针的干燥根中分离得到12个化合物,通过其一维或二维核磁共振谱、质谱和理化性质的解析并结合文献鉴定了它们的结构,分别为9-去甲氧基两面针碱(1)、8-dehydroxyl-buesgenine(2)、6β-hydroxymenthyldihydronitidine(3)、花椒木精(4)、rhoifoline B(5)、两面针碱(6)、白屈菜红碱(7)、博落回碱(8)、白鲜碱(9)、γ-崖椒碱(10)、茵芋碱(11)和鹅掌楸碱(12)。其中,化合物1和2为新结构化合物。通过MTT法,首次评价了化合物1~12对黑色素瘤细胞WM9增殖的抑制活性,并发现化合物3和6对WM9细胞的增殖表现出较强的活性,半数抑制浓度(IC50)值分别为1.936μM和0.880μM,研究结果为抗肿瘤药物候选药物分子的发现提供了科学依据。There are twelve compounds isolated from the ethanol extraction part of Zanthoxylum nitidum,which was traditional Chinese medicine,and the structure of these compounds were characterized through 1D,2D NMR spectrometry,mass spectrometry,as well as physicochemical properties research experiment.They were 9-demethoxynitidine(1),8-dehydroxyl-buesgenine(2),6β-hydroxymenthyldihydronitidine(3),zanthoxyline(4),rhoifoline B(5),nitidine(6),chelerythrine(7),bocconoline(8),dictamnine(9),γ-fagarine(10),skimmianine(11),liriodenine(12).Among them,compound 1 and compound 2 were found from Zanthoxylum nitidum for the first time and never been reported.The proliferation inhibition activity of compounds 1-12 against melanoma cells WM9 was evaluated for the first time by MTT method.The results showed that compound 3(IC50=1.936μM)and compound 6(IC50=0.880μM)have potent anti-proliferation activity of WM9 cells.This research has provided promising candidate molecules for the discovery of anticancer drugs.

关 键 词：滇产两面针 芸香科 生物碱 苯骈菲啶类 黑色素瘤 

分 类 号：R284.1[医药卫生—中药学]