A validated HPTLC method for quantitative analysis of morin in Maclura cochinchinensis heartwood  

作　　者：Savita Chewchinda Sumet Kongkiatpaiboon 

机构地区：[1]Department of Food Chemistry,Faculty of Pharmacy,Mahidol University,Bangkok 10400,Thailand [2]Drug Discovery and Development Center,Office of Advanced Science and Technology,Thammasat University(Rangsit Campus),Pathum Thani 12121,Thailand

出　　处：《Chinese Herbal Medicines》2020年第2期200-203,共4页中草药（英文版）

基　　金：financial supported by Thammasat University Research Fund under the TU Research Scholar,Contract No.2/29/2561。

摘　　要：Objective:The study was conducted to develop and validate a high performance thin-layer chromatography(HPTLC)-densitometric method for the quantitative analysis of morin in Maclura cochinchinensis heartwood collected from different locations in Thailand.Methods:HPTLC analysis was performed on an aluminium sheet of silica gel 60 F254 using toluene:ethyl acetate:formic acid(36:12:7,volume percent)as a mobile phase.The densitometric scanning was performed at the wavelength 410 nm.HPTLC method was validated according to ICH guideline.Results:The proposed HPTLC method showed acceptable validation parameters.The content of morin in M.cochinchinensis heartwood collected from eight different provinces in Thailand were in the ranges of1.53%-2.73%.Conclusion:The simple and sensitive HPTLC method was successfully developed and validated for determination of morin in M.cochinchinensis heartwood.The proposed HPTLC method was found to be simple,fast and inexpensive,and can be used for the routine quality control of raw materials.

关 键 词：HPTLC Maclura cochinchinensis(Lour.)Corner MORIN 

分 类 号：R284.1[医药卫生—中药学]