绿光空间光干涉显微镜技术  

Spatial-Light Interference Microscope Technology Using Green-Light

作　　者：郑明杰[1] 李志芳[1] Zheng Mingjie;Li Zhifang(Key Laboratory of Opto.Electronic Science and Tech nology for Medicine of Ministry of Education,Fujian Prooincial Key Laboratory of Photonics Technology,Fujian Normal University,Fuzhou,Fujian 350007,China)

机构地区：[1]医学光电科学与技术教育部重点实验室,福建省光子技术重点实验室,福建师范大学,福建福州350007

出　　处：《激光与光电子学进展》2020年第13期271-281,共11页Laser & Optoelectronics Progress

基　　金：国家自然科学基金(61875038)。

摘　　要：首先从理论上推导准相干或宽带光照明下空间光干涉光场的分布,由此可得准相干或宽带光照明下的四步相移法。其次,通过搭建绿光空间光干涉显微镜(SLIM)系统,以直径为6μm的聚苯乙烯微球分散于显微镜物镜用油模拟细胞的微环境。最后,利用4幅SLIM图像成功重建出油浸微球的相位分布,平均半径相对误差为6.5%,光程差体积相对误差为8.4%。由于相干光的四步相移法和宽带光的四步相移法重建结果相差较小,因此,在追求成像速度的场合且细胞厚度较小时,可不对四步相移法进行修正,以加快相位重建的速度。In this study,we first derive the light field distribution for the spatial interference with quasi-coherent or broadband light illumination,so as to obtain a four-step phase shift corrected method for such an illumination.Second,we construct a spatial interference microscope system that uses green light and diluted 6-μm polystyrene microsphere into the microscopic objective oil to simulate microenvironment of a cell.Finally,using four of the spatial-interference images,we reconstruct the phase distribution of the oil-immersed microspheres.The relative error in the microsphere radius is 6.5%,and the relative error of optical path difference volume of the oil-immersed microsphere is 8.4%.By comparing these results with those achieved by the traditional four-step phase shift method,the improvement can be neglected.Therefore,in situations where the imaging speed is important and the observed cell is sufficiently thin,it is not necessary to use the corrected four-step phase-shift method to increase the imaging speed.

关 键 词：显微 空间光干涉显微镜 细胞 聚苯乙烯微球 光程差 四步相移法 

分 类 号：O436.1[机械工程—光学工程]