响应面法优化超声辅助提取白簕多糖工艺研究  

Research on optimization of ultrasound-assisted extraction of total polysaccharide from Acanthopanax trifoliatus using response surface methodology

作　　者：郭鑫磊 赵雨晴 徐汉 杨娜 王宝庆[1] GUO Xin-lei;ZHAO Yu-qing;XU Han;YANG Na;WANG Bao-qing(Key Laboratory of Drug Research on Prevention and Treatment of Geriatric Diseases,Key Laboratory of Ministry of Finance and Local Co-construction,School of Pharmacy,Harbin University of Commerce,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学药学院,省预防治疗老年病药物研究重点实验室,财政部中央与地方共建实验室,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2020年第4期398-403,共6页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：哈尔滨商业大学青年创新人才支持项目(2016QN077);哈尔滨商业大学博士科研启动基金项目(13DL020);哈尔滨商业大学大学生创新训练项目(201810240036).

摘　　要：以白簕粉末为原料,用超声波细胞破碎法对多糖成分进行提取.选取有机溶剂种类、料液比、溶剂体积分数、提取时间和超声功率作为考察因子,用所含多糖成分的提取率作为目标,进行单因素实验,然后进行响应面分析.发现白簕中多糖的最好的提取方法是为料液比1∶20(g/mL)、乙醇体积分数72%、提取时间15 min、超声功率100 W,提取率的预测值为6.89%,实际值为6.85%,数值接近.因此,响应面优化的工艺稳定可靠,为白簕的综合利用和产业化开发提供理论支持.Polysaccharide was extracted fromAcanthopanax trifoliatuspowder by ultrasonic cell breaking method. The kinds of organic solvents,the solid-liquid ratio,the concentration of extraction solvent,the extraction time and the ultrasonic power were selected as the influencing factors,and the extraction rate of polysaccharide was taken as the response value to carry out the single factor experiment. On this basis,the box Behnken design and response surface analysis were carried out. The results showed that the best extraction conditions of Acanthopanax trifoliatus(L.)Merr. polysaccharide were:solid-liquid ratio 1 ∶20(g/mL),the volume fraction of ethanol was 72%,extraction time 15 minutes,ultrasonic power 100 W,and the predicted value of extraction rate was 6. 89%,the real value was 6 85%.Therefore,the process of response surface optimization was stable and reliable,which provided theoretical support for the comprehensive utilization and industrial development of Acanthopanax trifoliatus.

关 键 词：白簕 多糖 超声波辅助提取 单因素 响应面法 工艺研究 提取率 

分 类 号：R932[医药卫生—生药学]