孕期手机辐射暴露后大鼠仔鼠前额叶皮质多巴胺D1亚型受体的表达变化  

Changes on dopamin D receptor expression in the prefrontal cortex of offspring rats exposed to mobilephone radiation during pregnancy

作　　者：李容 王凌星[2] 刘良财 张燕琴 LI Rong;WANG Ling-xing;LIU Liang-cai;ZHANG Yan-qin(Department of Anatomy,Institute of Basic Medical Sciences,Quanzhou Medical College,Fujian Quanzhou 362000,China;Department of Neurology,the Second Affiliated Hospital of Fujian Medical University,Fujian Quanzhou 362000,China;Department of Traffic Police Brigade,Public Security Bureau of Jinjiang,Fujian Jinjiang 362200,China)

机构地区：[1]泉州医学高等专科学校基础医学部人体解剖学教研室,福建泉州362000 [2]福建医科大学附属第二医院神经内科,福建泉州362000 [3]晋江市公安局交警大队,福建晋江362200

出　　处：《解剖学报》2020年第4期491-494,共4页Acta Anatomica Sinica

基　　金：“国家示范性院校建设”重点项目(XJ1309);泉州市科技局项目(2014Z75)。

摘　　要：目的探讨孕期手机辐射暴露对大鼠仔鼠前额叶皮质多巴胺D1亚型受体(D1DR)表达的影响。方法30只妊娠大鼠随机分为3组:正常对照组、低剂量辐射组和高剂量辐射组。各组新生鼠随机分别留养80只,分别于出生后第1、10、40和60天取脑前额叶皮质待检。采用免疫组织化学方法检测D1DR表达的位置及强度,采用Real-time PCR方法检测D1DR mRNA的相对表达。结果实验结果显示,D1DR表达在神经元的胞质、胞膜和轴突,两个辐射组的表达强度均显著低于正常对照组(P<0.05),且高剂量辐射组显著低于低剂量辐射组(P<0.05);Real-time PCR结果与免疫组织化学方法实验结果一致。结论孕期手机辐射暴露可导致仔鼠前额叶皮质的D1DR表达水平降低。Objective To observe the changes of dopamin D1 receptor(D1 DR)expression in the prefrontal cortex of offspring rats exposed to mobilephone radiation during pregnancy.Methods Thirty pregnant rats were randomly divided into three groups namely normal control group,low-dose radiation group and high-dose radiation group.Eighty newborn rats were randomly kept in each group.The offspring rats’prefrontal cortices were collected at day 1,day 10,day 40 and day 60 after birth.The position and intensity of D1 DR were detected by immunohistochemistry,and the relative expression of D1 DR mRNA was detected by Real-time PCR.Results The experimental result showed that D1 DR was expressed in the cytoplasm,membrane and axon of neurons.The expression intensity of D1 DR of the two radiation groups was significantly lower than that of the normal control group(P<0.05)and the expression intensity on D1 DR of the high-dose radiation group was significantly lower than that of the low-dose radiation group(P<0.05).The result of Real-time PCR was consistent with those of immunohistochemistry.Conclusion Exposure to mobile phone radiation during pregnancy could result decreased D1 DR expression level in the prefrontal cortex of offspring rats.

关 键 词：孕期 手机辐射 多巴胺D1亚型受体 实时定量聚合酶链反应 大鼠仔鼠 

分 类 号：R715.3[医药卫生—妇产科学]