补阳还五汤调控脊髓损伤后NLRP3炎症体介导炎症反应的机制  

Mechanism of Buyang-Huanwu Decoction regulating NLRP3 inflammasome mediated inflammation after spinal cord injury

作　　者：丁洁[1] 徐天睿 范筱 Ding Jie;Xu Tianrui;Fan Xiao(Rehabilitation and Physiotherapy Department of Traditional Chinese Medicine,the 73rd Army Hospital of the Chinese People's Liberation Army,Xiamen 361003,China;No.1 Department of Orthpaedics Center,the 73rd Army Hospital of the Chinese People's Liberation Army,Xiamen 361003,China;Department of Traditional Chinese Medicine,Qingdao Municipal Hospital,Qingdao 266011,China;Fujian Universities and Colleges Engineering Research Center of Marine Biopharmaceutical Resources,Xiamen 361023,China)

机构地区：[1]中国人民解放军陆军第73集团军医院中医康复理疗科,厦门361003 [2]中国人民解放军陆军第73集团军医院骨科中心一区,厦门361003 [3]山东省青岛市市立医院中医科,266011 [4]海洋生物医药资源福建省高校应用技术工程中心,厦门361023

出　　处：《国际中医中药杂志》2020年第6期552-556,共5页International Journal of Traditional Chinese Medicine

基　　金：山东省中医药科技发展计划项目(2019-0614);海洋生物医药资源福建省高校应用技术工程中心资助项目(XMMC-MBS201903)。

摘　　要：目的研究补阳还五汤对脊髓损伤后NOD样受体家族3(NOD-like receptors,NLRP3)炎症体介导炎症的作用机制。方法将48只大鼠按随机数字表分为假手术组、模型组、补阳还五汤组,每组16只。模型组和补阳还五汤组采用脊髓打击器击打脊髓,建立脊髓损伤模型。补阳还五汤组灌胃补阳还五汤12.6 g/(kg·d),假手术组与模型组灌胃等体积无菌生理盐水,1次/d,连续给药3 d。采用尼氏染色法观察各组脊髓组织神经细胞形态,免疫组化染色法检测脊髓组织NLRP3表达,ELISA法检测血清IL-1β、IL-18水平,Western Blot检测脊髓组织活化型半胱氨酸天冬氨酸蛋白酶1(cleaved Systeinyl aspartate-specific protease,cleaved-Caspase1)表达。结果与模型组比较,补阳还五汤组脊髓组织NLRP3阳性表达平均光密度值[(0.54±0.04)比(0.78±0.06)]降低(P<0.05),血清IL-1β[(43.66±1.21)pg/ml比(67.64±2.43)pg/ml]、IL-18[(49.43±3.88)pg/ml比(65.87±2.53)pg/ml]水平降低(P<0.05),脊髓组织cleaved-Caspase1蛋白[(0.63±0.02)比(0.79±0.07)]表达降低(P<0.05)。结论补阳还五汤通过抑制脊髓损伤后NLRP3炎症体表达,减少Caspase1活化,从而减轻炎症反应。Objective To observe the mechanism of Buyang-Huanwu Decoction on inflammation mediated by NOD-like receptors(NLRP3)inflammasome.Methods A total of 48 rats were divided into sham operation group,model group and Buyang-Huanwu Decoction group,16 in each group according to the random number table method.The model group and the Buyang-Huanwu Decoction group used spinal cord blowers to hit the spinal cord to establish a spinal cord injury model.The Buyang-Huanwu Decoction group was gavaged with Buyang-Huanwu Decoction 12.6 g/(kg•d),and the sham operation group and the model group were gavaged with equal volume of sterile saline once a day for 3 days.Nissl staining was used to observe the neuronal morphology of spinal cord tissues in each group.Immunohistochemical staining was used to detect the expression of NLRP3 in spinal cord tissues.ELISA was used to detect the levels of serum IL-1βand IL-18.Western blot was used to detect the expression of cleaved systeinyl aspartate-specific protease(cleaved-Caspase1).Results Compared to the model group,the average optical density of NLRP3 positive expression(0.54±0.04 vs.0.78±0.06)in Buyang-Huanwu decoction group significantly decreased(P<0.05);the content of IL-1β(43.66±1.21 pg/ml vs.67.64±2.43 pg/ml)and IL-18(49.43±3.88 pg/ml vs.65.87±2.53 pg/ml)in serum of the Buyang-Huanwu decoction group significantly decreased(P<0.05);the expression of cleaved caspase 1 protein(0.63±0.02 vs.0.79±0.07)in Buyang-Huanwu decoction group significantly decreased(P<0.05).Conclusions The Buyang-Huanwu Decoction can reduce the inflammatory response by inhibiting the expression of NLRP3 inflammasome after spinal cord injury,by reducing the activation of Caspase1.

关 键 词：补阳还五汤 脊髓损伤 炎症 半胱氨酸天冬氨酸蛋白酶1 大鼠 

分 类 号：R285.5[医药卫生—中药学]