薄荷抗呼吸道合胞病毒活性及机制研究  

Study on the anti-RSV activity and mechanism of Mentha haplocalyx Briq.

作　　者：史晨晓 徐佳馨 郭浩 周长征[1] Shi Chenxiao;Xu Jiaxin;Guo Hao;Zhou Changzheng(School of Pharmacy,Shandong University of Traditiongal Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学药学院,济南250355

出　　处：《国际中医中药杂志》2020年第6期562-566,共5页International Journal of Traditional Chinese Medicine

摘　　要：目的探索薄荷不同提取方法对呼吸道合胞病毒(respiratory syncytial virus,RSV)的体外抑制作用及有效部位的抗病毒机制。方法采用细胞体外病毒感染模型,通过观察细胞病变效应法检测薄荷的体外抗RSV作用。以体外抗RSV的治疗指数(therapeutic index,TI)为指标,对薄荷提取方法及大孔吸附树脂进行筛选,从而确定最佳的提取方法,并以最佳大孔吸附树脂进行分离纯化,用不同浓度的洗脱液进行洗脱,确定薄荷抗RSV的有效部位。以不同给药方式进行干预,探讨其抗病毒作用机制。结果薄荷水提醇沉后的上清液对RSV的抑制作用最强,TI值为37.58;HPD100树脂对上清液的分离纯化效果最佳,其中25%乙醇洗脱部位效果最佳,TI值为57.8。理化鉴别反应确定其有效物质为多酚类物质;有效部位与病毒混合后,再进行抗病毒实验效果最佳。结论薄荷水提醇沉上清液经HPD100吸附后,25%乙醇洗脱液的抗RSV作用最佳,具有预防和治疗作用。Objective To explore the inhibitory effects of Mentha haplocalyx Briq.With different extraction methods on respiratory syncytial virus(RSV)in vitro and antiviral mechanism of it seffective parts.Methods By using the virus infection model in vitro to detect the cytopathic effect(CPE)of the anti-RSV effect of Mentha haplocalyx Briq.in vitro.Make the anti-RSV therapeutic index(TI)in vitroas the index to screen the extraction method of mint and the macro-porous adsorption resin,so as to determine the best extraction method,and the best macroporous adsorption resin was used for separation and purification.Elution with different concentrations of eluent was used to determine the effective parts of Mentha haplocalyx Briq.resisting RSV.The antiviral mechanism was analyzed by different administration methods.Results The supernatant of Mint after water extraction and alcohol precipitation had the strongest inhibitory effect on RSV,with TI value of 37.58;HPD100 resin had the best separation and purification effect on the supernatant,and 25%ethanol elution site had the best effect,and the TI value was 57.8.The effective substances were determined to be polyphenols by physicochemical identification reaction.After the effective parts are mixed with the virus,the antiviral experiment is the best.Conclusions The supernatant of mint after water extraction and alcohol precipitation was adsorbed by HPD100,and 25%ethanol eluent had the best anti-RSV effect,with preventive and therapeutic effects.

关 键 词：薄荷 抗病毒药(中药) 提取法 大孔吸附树脂 治疗指数 

分 类 号：R285[医药卫生—中药学]