胰腺移植动物模型新选择——门静脉回流胰腺腹腔移植小鼠模型  

A New Choice of Pancreas Transplantation Animal Model--Portal Venous Drainage(PVD)Model in Mice

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作  者:史惠中 夏俊[1] 陈云娇 贾冉[1] 俞广进[1] 熊奇如[1] SHI Hui-zhong;XIA Jun;CHEN Yun-jiao(The First Affiliated of Anhui Medical University,Hefei 230022,China)

机构地区:[1]安徽医科大学第一附属医院,合肥230022

出  处:《肝胆外科杂志》2020年第3期230-233,236,共5页Journal of Hepatobiliary Surgery

摘  要:目的胰腺移植是1型糖尿病和2型糖尿病的推荐治疗方法。在胰腺移植术式中,移植胰腺静脉血经门静脉回流对患者预后有益存在争议。因此,需要一种符合门静脉回流的胰腺动物模型来进行相关的基础研究。小鼠在实验室中被广泛应用,在胰腺免疫损伤和内分泌研究方面具有优势,但至今没有经门静脉回流的小鼠胰腺移植模型。建立经门静脉回流的胰腺移植小鼠模型。方法供体胰腺保留含有肠系膜上动脉与腹腔动脉的一段腹主动脉和门静脉。受体游离脾静脉和腹主动脉,采用插管技术连接移植胰腺和受体的血管。结果我们利用显微外科技术,成功建立了一种新的经门静脉回流胰腺移植小鼠模型。通过30组小鼠验证,移植后生存率为93.3%(28/30),胰腺有功能率为92.9%(26/28),模型的其他相关指标(生存时间、手术时间、冷/热缺血时间、移植胰腺功能)也令人满意。结论该模型具有可重复性和可靠性,可用于对门静脉回流胰腺移植手术探讨、胰腺灌注保存、胰腺缺血再灌注损伤和胰腺免疫损伤的研究。Objective Pancreas transplantation(PTx)is the recommended treatment for type 1 diabetes and selected cases of Type 2.It’s reported that the operation of PVD is benefit in PTx which is in dispute.A high-quality animal pancreas transplantation model with physiological blood flow characteristics is needed in basic research on PTx of PVD.Mice are widely used in laboratory and have advantages in studying pancreatic immune injury and endocrine but we did not find a model of PTx with PVD in mice.To establish a model of PTx with PVD in mice.Method The donor pancreas retains the abdominal aorta between the superior mesenteric artery and the celiac artery and the portal vein.Intubation technique was used to connect the blood vessels including abdominal aorta,splenic vein and portal vein.Result We established a new model of PTx in mice with PVD(Schematic diagram)by means of microsurgical technique.The survival rate was 93.3%(28/30)and the functional rate was 92.9%(26/28).Demonstrated in diabetic mice,other relevant indicators(the survival time,the operation time,the cold/warm ischemia time and the transplanted pancreas function)of the model are also satisfactory.Conclusion This model is replicable and reliable,and can be used in the studies of PVD operation of PTx,preservation of pancreas perfusion,pancreatic ischemia reperfusion injury and pancreatic immune injury.

关 键 词:小鼠 动物模型 胰腺移植 门静脉回流 

分 类 号:R659[医药卫生—外科学]

 

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