PD-1在4NQO诱导的小鼠口腔黏膜鳞状细胞癌中表达变化  

Alteration of PD-1 expression in mice oral squamous cell carcinoma induced by 4-nitroquinoline-1-oxide

作　　者：张柏林 李岩 朱雪琴[2] 蒋英英[1,2] 陈万涛 ZHANG Bolin;LI Yan;ZHU Xueqin;JIANG Yingying;CHEN Wantao(Department of Oral and Maxillofacial Surgery,School of Stomatology,Weifang Medical University,Weifang 261000,China)

机构地区：[1]潍坊医学院口腔医学院口腔颌面外科,山东潍坊261000 [2]上海交通大学医学院附属第九人民医院口腔颌面-头颈肿瘤科,上海市口腔医学研究所及上海市口腔医学重点实验室,国家口腔疾病临床研究中心,上海200011

出　　处：《口腔医学》2020年第7期593-596,共4页Stomatology

基　　金：国家自然科学基金(81472515);上海市科学技术委员会项目(18DZ2291500)。

摘　　要：目的研究程序性死亡蛋白1(programmed cell death1,PD-1)在小鼠口腔黏膜鳞状细胞癌微环境中的变化和可能作用。方法利用化学致癌剂4-硝基喹啉-1-氧化物(4-nitroquinoline-1-oxide,4NQO)饮水诱导建立C57小鼠口腔黏膜鳞癌动物模型。对小鼠模型口腔黏膜肿瘤病变部位进行组织病理学检测和分析;通过Real-time PCR和流式细胞术检测免疫检查点PD-1基因和蛋白的表达水平。结果在暴露4NQO 28周后,成功诱导小鼠发生口腔黏膜鳞癌。组织病理学结果显示,小鼠发生的肿瘤为口腔黏膜鳞癌。Real-time PCR和流式细胞术检测结果表明,小鼠舌部黏膜肿瘤组织和脾脏中的CD4^+T细胞,PD-1基因和蛋白表达都明显上调。结论免疫检查点PD-1在小鼠口腔黏膜鳞癌发生中出现明显升高,PD-1与口腔黏膜鳞癌的发展可能相关。Objective To identify the changes and potential roles of immune checkpoint PD-1 in oral squamous cell carcinoma(OSCC)microenvironment.Methods The oral squamous cell carcinoma model of C57 mice was induced and established by potable water containing 4-nitroquinoline-1-oxide(4 NQO).Lesion location of the oral squamous cell carcinoma of the mice model was detected and analyzed by histopathology.Real-time PCR and flow cytometry were used to detect the expression level of PD-1 gene and protein.Results OSCC model was successfully induced by exposing to 4 NQO after 28 weeks.Histopathological evaluation showed that oral mucosal squamous cell carcinoma occurred in mice.Real-time PCR and flow cytometry showed that PD-1 gene and protein expression in CD4^+T cell was significantly up-regulated in tongue mucosal tumor tissues and spleen.Conclusion PD-1 shows significant up-regulation in mice OSCC and is possibly related to the development of OSCC.

关 键 词：小鼠口腔黏膜 4-硝基喹啉-1-氧化物 口腔鳞状细胞癌 PD-1 

分 类 号：R839.85[医药卫生—航空、航天与航海医学]