Effect of Korean Magnolia obovata Extract on Platelet-Derived Growth Factor-Induced Vascular Smooth Muscle Cells  

作　　者：Hyunjhung Jhun Suji Baek Jinwoo Kim Kang-Pa Lee Hun-Young Park Won-Hwan Park Kiwon Lim Jisu Kim 

机构地区：[1]Technical Assistance Center,Korea Food Research Institute,Jeonbuk(55365),Republic of Korea [2]Department of Medical Science,School of Medicine,Konkuk University,Seoul,Republic of Korea [3]Department of Bio-Science,College of Natural Science,Dongguk University,Gyeongbuk(38066),Republic of Korea [4]Physical Activity&Performance Institute,Konkuk University,Seoul(05029),Republic of Korea [5]Department of Diagnostic,College of Korean Medicine,Dongguk University Goyang,Gyeonggi-do(10326),Republic of Korea

出　　处：《Chinese Journal of Integrative Medicine》2020年第9期677-682,共6页中国结合医学杂志（英文版）

摘　　要：Objective:To investigate the effects of Korean Magnolia obovata crude extract(KME)on plateletderived growth factor(PDGF)-BB-induced proliferation and migration of vascular smooth muscle cells(VSMCs).Methods:KME composition was analyzed by high-performance liquid chromatography(HPLC).VSMCs were isolated from the aorta of a Sprague-Dawley rat,incubated in serum free-Dulbecco's modified Eagle's medium in the presence or absence of KME(10,30,100,and 300(xg/mL),then further treated with PDGF-BB(10 ng/mL).VSMC proliferation was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and VSMC migration was determined using the Boyden chamber and scratch wound healing assays.Western blot analysis was used to detect phosphorylation of extracellular signal-regulated protein kinases 1 and 2(p-ERK1/2),protein kinase B(p-Akt),and stress-activated protein kinase/c-Jun NH2-terminal kinase(p-SAPK/JNK).The antimigration and proliferation effects of KME were tested using aortic sprout outgrowth.Results:The HPLC analysis identified honokiol(0.45 mg/g)and magnolol(0.34 mg/g)as the major components of KME.KME(30,100,and 300μg/m L)significantly decreased the proliferation and migration of PDGF-BB-stimulated(10 ng/mL)VSMCs and the PDGF-BB-induced phosphorylation of EKR1/2,Akt,and SAPK/JNK(P<0.05).Furthermore,PDGF-BBinduced VSMCs treated with 300μg/m L of KME showed reduction in aortic sprout outgrowth.Conclusion:KME could inhibit abnormal proliferation and migration of VSMCs by down-regulating the phosphorylation of EKR1/2 and Akt.Thus,KME might be a functional food for preventing vascular disorders.

关 键 词：extracellular signal-regulated protein kinases 1 and 2 AKT platelet-derived growth factor-BB smooth muscle cell functional food 

分 类 号：R285.5[医药卫生—中药学]