小黑麦TwNA C01基因功能分析  

Analysis of TwNAC01 Gene Function from Triticale(×Triticosecale Wittm-ack)

作　　者：王萌 任丽彤[2] 凌悦铭 谷海涛 艾尼瓦尔·江 李召锋[1] 孔广超[1] Wang Meng;Ren Litong;Ling Yueming;Gu Haitao;Ainiwaer·Jiang;Li Zhaofeng;Kong Guangchao(The Key Laboratory of Oasis Eco-Agriculture,Xinjiang Production and Construction Corps,Agriculture College of Shihezi University,Shihezi,832003;School of Chemistry and Chemical Engineering of Shihezi University,Shihezi,832003)

机构地区：[1]石河子大学农学院,新疆生产建设兵团绿洲生态农业重点实验室,石河子832003 [2]石河子大学化学化工学院,石河子832003

出　　处：《分子植物育种》2020年第16期5221-5229,共9页Molecular Plant Breeding

基　　金：国家自然基金项目(31860376,31360333)资助。

摘　　要：NAC是一类调控植物发育、衰老、生物与非生物逆境以及在激素反应方面具有重要作用的转录因子。为解析小黑麦TwNAC01基因的初步功能,本研究以从六倍体小黑麦中克隆到NAC转录因子基因TwNAC01为基础,构建了过表达载体pCAMBIA1300-35S-Tw NAC01,并通过蘸花法转化拟南芥,获得转基因拟南芥株系,对转基因与野生型拟南芥在干旱胁迫下的该基因表达量与相关生理指标进行了检测。结果表明,在干旱胁迫下,TwNAC01基因在拟南芥根部表达量显著高于叶部,同时在转Tw NAC01基因植株根和叶部的表达量均显著高于野生型及转空载体拟南芥。转TwNAC01基因拟南芥叶片在干旱胁迫下失水率较转空载体和野生型拟南芥降低,其电导率、H2O2和MDA含量较转空载体及野生型株系显著降低,但叶片含水量在转TwNAC01基因株系与较转空载体及野生型株系间差异不显著,而转Tw NAC01基因拟南芥根系长度分别是野生型和转空载体拟南芥根系长度的1.5倍和1.2倍,差异显著。可见Tw NAC01在拟南芥根部优势表达,其优势表达促进了根系的伸长,提高了拟南芥抗旱能力。本研究对于理解小黑麦的抗旱分子机制提供了重要依据,也为小黑麦抗旱育种奠定了分子基础。NAC is a class of transcription factors that play important roles in regulating plant development,senescence,biotic and abiotic stresses,and hormonal responses.In order to analyze the function of TwNAC01 gene from triticale,the gene over-expression vector pCAMBIA1300-35S-TwNAC01 was constructed based on the cloned NAC transcription factor gene TwNAC01 in triticale,and the over-expression vector was transformed into Ara-%bidopsis thaliana by dipping flowers in this study.The TwNAC01 expression characteristic and physiological in-dexes of the transgenic Arabidopsis thaliana and wild type under drought stress were detected.The results showed that under drought stress,the expression of TwNAC01 in transgenic Arabidopsis roots was significantly higher than that in leaves,and the expression of TwNAC01 in roots and leaves was significantly higher than that in wild-type and Arabidopsis plants with empty vector.The water loss rate of transgenic Arabidopsis thaliana leaves was slower under drought stress than transgenic vector and wild type Arabidopsis plants and Arabidopsis thaliana with empty vector.The electrical conductivity and content of H2O2 and MDA in transgenic Arabidopsis thaliana lines were lower than that of transgenic Arabidopsis thaliana with empty vector and wild type.The root length of transgenic Arabidopsis thaliana was 1.5 times and 1.2 times longer than that of wild type and transgenic Arabidopsis with vector,respectively.It can be concluded that TwNAC01 express dominantly in the roots of Arabidopsis thaliana,and it can promotes the Arabidopsis roots elongating,and enhance the Arabidopsis drought resistance.This study lays the foundation to understand the molecular mechanism of drought resistance of triticale,and provides an im-portant basis for drought-resistant breeding of triticale.

关 键 词：小黑麦(×Triticosecale Wittmack) TwNA C01 NAC转录因子 表达载体 

分 类 号：S512.4[农业科学—作物学]