非生物胁迫激活ABA途径ACO3基因的表达  

Abiotic Stress Activate the Expression of ACO3 Gene in the ABA Pathway

作　　者：金太成[1] 郎晨婧 孟大伟[1] 王悦 吴艳菊 杨丽萍[1] Jin Taicheng;Lang Chenjing;Meng Dawei;Wang Yue;Wu Yanju;Yang Liping(School of Life Science,Jilin Normal University,Siping,136000)

机构地区：[1]吉林师范大学生命科学学院,四平136000

出　　处：《分子植物育种》2020年第16期5330-5334,共5页Molecular Plant Breeding

基　　金：国家青年科学基金项目(31301043);吉林省教育厅科研项目(JJKH20191013KJ)共同资助。

摘　　要：DNA甲基化在植物生长发育和应答非生物胁迫过程中起重要作用。本研究揭示了ABA途径相关基因ACO3应答非生物胁迫的分子机制。定量PCR (quantitative PCR, qPCR)的检测结果表明用野生型拟南芥(Col-0)作对照,被干旱、低温和盐处理的拟南芥植物中ACO3基因表达水平显著增加。重亚硫酸盐测序的数据分析表明非生物胁迫能够诱导ACO3启动子DNA去甲基化并激活该基因的表达。RNA介导的DNA甲基化(RNA directed DNA methylation, RdDM)途径突变体ago4和dcl3中ACO3基因表达水平明显增加,暗示RdDM途径在调控ACO3基因应答非生物胁迫过程中起重要作用。DNA methylation plays an important role in the plant growth and development and in the process of response to abiotic stress.In this paper,we discovered the molecular mechanism that ACO3 gene in ABA pathway responses to abiotic stress.The detection results of quantitative PCR(qPCR)revealed that the expression level of ACO3 gene was significantly increased in Arabidopsis plants treated by drought,cold and salt stress,with wild-type Arabidopsis(Col-0)plants were used as controls.The data analysis of bisulfite sequencing showed that abiotic stress can induce DNA demethylation of the ACO3 promoter and activated the gene expression.The expression level of ACO3 was clearly increased in the RNA directed DNA Methylation(RdDM)mutants ago4 and dcl3,suggesting that RdDM plays an important role in the regulation process of the ACO3 gene response to abiotic stress.

关 键 词：非生物胁迫 RNA介导的DNA甲基化 拟南芥 

分 类 号：Q943.2[生物学—植物学]