基于荧光SSR标记的鳄梨种质资源遗传多样性  

Genetic Diversity Analysis of Germplasm Resources of Persea americana Mill.Based on SSR Fluorescent Marker

作　　者：董美超[1] 岳建强[1] 李进学[1] 杨帆[1] 周东果[1] 王绍华[1] 龙春瑞[1] 郭莉娜 高俊燕[1] 杨恩聪[1] Dong Meichao;Yue Jianqiang;Li Jinxue;Yang Fan;Zhou Dongguo;Wang Shaohua;Long Chunrui;Guo Li'na;Gao Junyan;Yang Encong(Institute of Tropical and Subtropical Cash Crops,Yunnan Academy of Agricultural Sciences,Baoshan,678000)

机构地区：[1]云南省农业科学院热带亚热带经济作物研究所,保山678000

出　　处：《分子植物育种》2020年第16期5403-5410,共8页Molecular Plant Breeding

基　　金：云南省农业基础研究联合专项暨院应用基础研究专项面上项目(2017FG001(-060))资助。

摘　　要：本试验对滇缅地区鳄梨种质资源遗传多样性进行了研究,为鳄梨种质资源保存、评价与利用提供了参考。本研究对采自中国云南德宏、保山、西双版纳、普洱、红河、缅甸克钦邦和掸邦地区共90份鳄梨资源利用毛细管电泳法进行荧光SSR-PCR产物检测,并利用所得结果评价鳄梨遗传多样性水平。7对SSR引物对90份鳄梨资源进行扩增,得到72个等位基因,不同引物扩增的等位基因差异较大,从5到14个不等,平均每个位点含10.29个等位基因。主要等位基因频率在0.238 9~0.811 1之间,平均值为0.434 9;观测杂合度在0.022 2~0.766 7之间,平均值为0.431 7;期望杂合度在0.314 1~0.877 0之间,平均值为0.692 7;多态性信息指数在0.273 2~0.859 9之间,平均值为0.653 5。UPMGA聚类分析表明,遗传相似系数介于0.1~1.0之间,平均值为0.46,在阈值0.41处可分为4大类群。本研究为新品种选育促进产业健康发展和开发利用该地区独特鳄梨资源提供帮助。The genetic diversity of avocado germplasm resources in Yunnan and Myanmar region was studied in this experiment,which provided reference for avocado germplasm resources conservation,evaluation and utiliza-tion.The genetic relationship of 90 avocado populations from Dehong,Baoshan,Xishuangbanna,Puer,Honghe of Yunnan,China and the Shan state,Kachin state of Burma were analyzed by capillary electrophoresis for fluore-scent SSR-PCR products,and genetic diversity were analyzed based on the results.7 pairs of SSR primers were used to amplify 90 avocado resources,and 72 alleles were obtained.The alleles amplified by different primers were different,ranging from 5~14.The average number of alleles per locus was 10.29.The major allele frequency ranged from 0.2389 to 0.8111 with the mean value of 0.4349.The observational heterozygosity ranged from 0.0222 to 0.7667 with the mean value of 0.4317.The expected heterozygosity ranged from 0.3141 to 0.8770 with the mean value of 0.6927.The polymorphism information content ranged from 0.2732 to 0.8599 with the mean value of 0.6535.UPMGA cluster analysis showed that the genetic similarity coefficient ranged from 0.1 to 1.0,with an average of 0.46,and all materials could be divided into four groups at the threshold of 0.41.This study could be helpful for the breeding of new varieties,promoting the healthy development of industry and exploiting and utilizing the unique avocado resources in this area.

关 键 词：鳄梨(Persea americana Mill.) SSR 聚类分析 遗传多样性 

分 类 号：S667.9[农业科学—果树学]