重症肺炎患儿NADH脱氢酶1、NADH脱氢酶3和味觉受体2家族成员43基因表达的意义  

Analysis of expression of NADH dehydrogenase 1,NADH dehydrogenase 3 and taste 2 receptor member 43 genes in severe pneumonia children

作　　者：刘阳 杨莉 王凌啸 高利真 Liu Yang;Yang Li;Wang Lingxiao;Gao Lizhen(Department of Pediatrics,Zhumadian Central Hospital,Zhumadian 463000,Henan Province,China;Department of Hematology and Oncology,Henan Children′s Hospital,Zhengzhou 450000,China;Department of Childhood Critical Care Medicine,Zhumadian Central Hospital,Zhumadian 463000,Henan Province,China)

机构地区：[1]驻马店市中心医院儿科,河南驻马店463000 [2]河南省儿童医院血液肿瘤科,郑州450000 [3]驻马店市中心医院儿童重症医学科,河南驻马店463000

出　　处：《中华实用儿科临床杂志》2020年第13期1019-1021,共3页Chinese Journal of Applied Clinical Pediatrics

摘　　要：目的研究重症肺炎患儿NADH脱氢酶1(ND1)、NADH脱氢酶3(ND3)和味觉受体2家族成员43(TAS2R43)基因表达情况及与儿童重症肺炎的关系。方法选取2016年5月至2018年5月驻马店市中心医院儿童重症监护病房住院的儿童重症肺炎患儿30例为重症肺炎组,选取同期在本院体检健康的儿童25例为健康对照组。重症肺炎组男17例,女13例,年龄(5.30±1.69)岁;健康对照组男13例,女12例,年龄(4.96±1.31)岁。使用real-time PCR方法检测ND1、ND3和TAS2R43基因表达量,2-ΔΔCt法计算ND1、ND3和TAS2R43基因相对表达量。结果重症肺炎组ND1、ND3及TAS2R43 mRNA的循环阈(Ct)值分别为20.49±0.45、21.32±0.61和32.20±0.46,健康对照组分别为26.69±0.62、27.50±0.35和26.69±0.49,2组比较差异均有统计学意义(t=-14.02、-15.25、-14.19,均P<0.05)。2-ΔΔCt法计算重症肺炎组ND1、ND3和TAS2R43基因相对表达量是健康对照组的51.27、50.56和0.02倍。结论ND1、ND3和TAS2R43基因在重症肺炎患儿体内有异常表达,这3个基因可能与儿童重症肺炎关系密切。Objective To explore the association of expression of NADH dehydrogenase 1(ND1),NADH dehydrogenase 3(ND3)and taste 2 receptor member 43(TAS2R43)genes with severe pneumonia in children.Methods Thirty severe pneumonia children in the intensive care unit of Zhumadian Central Hospital from May 2016 to May 2018 were enrolled as the severe pneumonia group and 25 healthy children undergoing health examinations in Zhumadian Central Hospital over the same period were selected as the control group.The severe pneumonia group contained 17 boys and 13 girls,with an age of 5.30±1.69.The control group included 13 boys and 12 girls,with an age of 4.96±1.31.Expression of ND1,ND3 and TAS2R43 genes was detected by real-time PCR,and the relative expression of ND1,ND3 and TAS2R43 genes was calculated by 2-ΔΔCt method.Results The circulating threshold(Ct)values of ND1,ND3 and TAS2R43 mRNA in the severe pneumonia group were 20.49±0.45,21.32±0.61 and 32.20±0.46,respectively,and those in the healthy control group were 26.69±0.62,27.50±0.35 and 26.69±0.49,respectively.The difference between the 2 groups was statistically significant(t=-14.02,-15.25,-14.19,all P<0.05).The relative expre-ssion of ND1,ND3 and TAS2R43 genes calculated by the 2-ΔΔCt method in the severe pneumonia group was 51.27 folds,50.56 folds and 0.02 folds as many as that in the control group.Conclusions The expression of ND1,ND3 and TAS2R43 genes in severe pneumonia children is abnormal.These 3 genes may be closely related to severe pneumonia in children.

关 键 词：重症肺炎 NADH脱氢酶1 NADH脱氢酶3 味觉受体2家族成员43 基因表达 

分 类 号：R725.6[医药卫生—儿科]