碱性蓝B探针吸收光谱法测定美司那  

Determination of Mesna by Absorption Spectrometry with Basic Blue B as Probe

作　　者：刘晓红 江虹[1] 李晓彤 Liu Xiaohong;Jiang Hong;Li Xiaotong(College of Chemistry and Chemical Engineering,Yangtze Normal University,Chongqing 408100,China)

机构地区：[1]长江师范学院化学化工学院,重庆408100

出　　处：《化学世界》2020年第8期543-547,共5页Chemical World

基　　金：重庆市教委科技基金(No.KJ1401226);长江师范学院科技基金(No.2018CXX123)资助项目。

摘　　要：在pH 7.93的Britton-Robinson(BR)溶液中,美司那与碱性蓝B反应生成蓝色离子缔合物,光谱曲线上呈现1个强的正吸收峰和1个较强的负吸收峰,正吸收峰位为580 nm,表观摩尔吸光系数(κ)为7.04×10^4 L/(mol·cm),负吸收峰位为430 nm,表观摩尔吸光系数(κ)为1.03×10^4 L/(mol·cm),在此二个波长处,美司那的质量浓度为0~2.7 mg/L范围内服从朗伯-比尔定律,据此建立了测定美司那的正吸收光谱法和负吸收光谱法。该法用于市售美司那药物中美司那的测定,加标回收率为97.9%~98.9%,相对标准偏差(RSD)(n=5)为2.5%~2.8%。In Britton-Robinson(BR)solution(pH=7.93),mesna reacted with basic blue B to form an ionic association complex with both positive and negative absorption peaks on the spectrum.The positive absorption peak was located at 580 nm with an apparent molar absorptivity(κ)of 7.04×10^4 L/(mol·cm),while the negative absorption peak was located at 430 nm with an apparent molar absorptivity(κ)of 1.03×10^4 L/(mol·cm).At both wavelengths,the mass concentration of mesna obeyed the Lambert-Beer’s law in the range from 0 to 2.7 mg/L.Thus,both positive and negative absorption spectroscopy for the determination of mesna were established.The method has been applied to determine the content of mesna in commercially available mesna-containing drugs with an add standard recoveries of 97.9%~98.9%and relative standard deviation(RSD)(n=5)of 2.5%~2.8%.

关 键 词：美司那 碱性蓝B 正吸收 负吸收 吸收光谱法 

分 类 号：O657.3[理学—分析化学]