细胞松弛素D在TEG中对血小板制品凝血功能检测的应用研究  

Study on the application of cytochalasin D in platelet concentrates functional detection based on thromboelastography

作　　者：钟周琳 周燕 卢芳 李丽兰 吴国光 ZHONG Zhoulin;ZHOU Yan;LU Fang;LI Lilan;WU Guoguang(Nanning Institute of Transfusion Medicine,Nanning,Guangxi 530007,China)

机构地区：[1]广西壮族自治区南宁输血医学研究所,南宁530007

出　　处：《重庆医学》2020年第16期2663-2666,共4页Chongqing medicine

基　　金：广西壮族自治区自然科学基金面上项目(2016GXNSFAA380144);广西壮族自治区南宁市科学研究与技术开发计划项目(科技重大专项,20173117)。

摘　　要：目的应用细胞松弛素D(CD)抑制血小板参与凝血,建立血栓弹力图(TEG)检测血小板制品凝血功能的方法。方法通过优化实验条件,选择合适血小板浓度作为TEG检测血小板制品的工作浓度及合适浓度的CD抑制血小板,并与Functional Fibrinogen(FF)试剂盒比较抑制血小板的效果。应用CD抑制血小板,TEG检测获得血浆纤维蛋白原对最大振幅(MA)的贡献值MAfib,计算血小板贡献值MAplt;采用建立的方法检测30份单采血小板制品,获得正常参考范围。结果选择血小板浓度100×10^9/L作为TEG检测血小板制品的工作浓度,终浓度为3.0μmol/L的CD用于抑制血小板。CD可完全抑制浓度为50×10^9/L^500×10^9/L的血小板,而FF试剂盒无法完全抑制浓度大于200×10^9/L的血小板。检测30份不同献血者的单采血小板制品MA为(64.95±5.67)mm,MAfib为(24.65±5.42)mm,MAplt为(40.30±3.63)mm。结论本研究方法可应用于血小板制品功能的评估。Objective To establish a method for platelet concentrates aggregation based on thromboelastography(TEG)using cytochalasin D(CD)to inhibit the contribution of platelet to clot strength.Methods Appropriate platelet concentration and CD concentration were selected for TEG assay and platelet inhibition,while Functional Fibrinogen(FF)kit was applied for comparing with CD for the effect of platelet inhibition.MAfib of TEG was detected by using CD to inhibit platelet,then MAplt was calculated.A total of 30 PCs from different donors were detected to obtain the reference range of normal value of MAplt.Results Platelet 100×10^9/L as a working concentration was selected for detecting PCs by TEG,and CD with a final concentration of 3.0μmol/L was applied for platelet inhibition.CD could effectively inhibit 50×10^9/L-500×10^9/L platelet,while FF kit could not effectively inhibit platelet>200×10^9/L.The reference range of MA was(64.95±5.67)mm,MAfib was(24.65±5.42)mm,MAplt was(40.30±3.63)mm,respectively.Conclusion This method can be applied to evaluate the platelet function.

关 键 词：血栓弹力描记术 血小板 细胞松弛素D 血液凝固 最大振幅 

分 类 号：R558.2[医药卫生—血液循环系统疾病]