N-3-羰基十四烷酰基高丝氨酸内酯诱导番茄对灰霉病抗性机制初探  

The Exploration of the Effect of N-3-oxo-tetradecanoyl Homoserine Lactone on the Resistance to Botrytis cinerea on Tomato

作　　者：杨向云 刘方[2,3] 赵芊 宋水山[2,3] 张利平 YANG Xiangyun;LIU Fang;ZHAO Qian;SONG Shuishan;ZHANG Liping(College of Life Science, Hebei University, Baoding 071000, China;Institute of Biology, Hebei Academy of Sciences, Shijiazhuang 050000, China;Engineering Research Center for Microbial Control of Major Crop Diseases in Hebei Province, Shijiazhuang 050081, China)

机构地区：[1]河北大学生命科学学院,河北保定071000 [2]河北省科学院生物研究所,河北石家庄050000 [3]河北省主要农作物病害微生物控制工程技术研究中心,河北石家庄050081

出　　处：《华北农学报》2020年第4期169-176,共8页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31270880)。

摘　　要：为了探究细菌群体感应(QS)信号分子N-酰基高丝氨酸内酯(AHLs)在植物上诱导对于真菌病害的抵抗能力。以番茄为试验材料,以灰霉为病原指示菌,通过不同侧链长度和侧链修饰的AHLs信号分子预处理后接种病原菌,发现长链信号分子N-3-羰基十四烷酰基高丝氨酸内酯(3OC14-HSL)对灰霉有最佳的抗性效果。实时荧光定量PCR检测抗病基因发现,与未处理对照组相比,信号分子3OC14-HSL预处理能显著诱导接种灰霉病后的番茄体内与茉莉酸(JA)信号通路相应基因PI1、PI2上调;而与水杨酸信号(SA)通路相关的NPR1基因显著下调,PR1基因则没有显著变化。进一步通过DAB染色、过氧化氢含量测定以及过氧化物酶(POD)和超氧化物歧化酶(SOD)活性检测发现,3OC14-HSL预处理相比未处理对照组使得番茄产生大量活性氧,且可以保持较高的POD、SOD活性。这些结果表明,长链信号分子N-3-羰基十四烷酰基高丝氨酸内酯可以诱导番茄对灰霉的抗性,而且该抗性效果可能与茉莉酸信号路径相关。In order to determine the bacterial quorum sensing(QS)signaling molecule,N-acyl-homoserine lactones(AHLs)induce resistance to fungal diseases in plants.Tomato was used as the test material,Botrytis cinerea was used as the pathogen indicator bacteria,and AHLs signal molecules with different side chain length and side chain modification were pretreated to inoculate the pathogen bacteria,and the long chain signal molecule N-3-oxo-tetradecanoyl was found.3OC14-HSL had the best resistance to gray mold.Real-time quantitative PCR detection of disease resistance genes revealed that the signal molecule 3OC14-HSL pretreatment significantly induced up-regulation of the genes PI1 and PI2 in the tomato and jasmonic acid(JA)signaling pathway compared with the untreated control group;However,the NPR1 gene related to the salicylic acid signaling(SA)pathway was significantly down-regulated,but the PR1 gene was not significantly changed.Further DAB staining,determination of hydrogen peroxide content,and detection of peroxidase(POD)and superoxide dismutase(SOD)enzyme activities revealed that 3OC14-HSL pretreatment caused a large amount of reactive oxygen species in tomatoes compared to the untreated control group.3OC14-HSL also could maintain high POD,SOD activity.These results indicated that the long-chain signaling molecule N-3-oxo-tetradecanoyl-homoserine(3OC14-HSL)could induce resistance to gray mold of tomato,and the resistance effect might be related to the jasmonic acid signal pathway.

关 键 词：番茄 N-酰基高丝氨酸内酯 灰霉 抗性 

分 类 号：S432.4[农业科学—植物病理学]