荔枝核总黄酮对两种肝纤维化大鼠模型的作用比较  

Comparison of Total Flavone of Litchi Chinensis Sonn in Two Kinds of Hepatic Fibrosis Model of Rats

作　　者：成秋宸 覃雯 卓朗 赵永忠[3] 范丽雯[3] 林文斯 刘瑞彪 CHENG Qiuchen;QIN Wen;ZHUO Lang;ZHAO Yongzhong;FAN Liwen;LIN Wensi;LIU Ruibiao(National Engineering Laboratory of Southwest Endangered Medicinal Guangxi Botanic Garden of Medicinal Plants,Nanning 530023,China;Department of Infectious Diseases,the First Affiliated Hospital of Guangxi Medical University,Nanning 530023,China;Department of Gastroenterology,the First Affiliated Hospital of Guilin Medical University,Guilin 541001,China;Clinical Laboratory,the First Affiliated Hospital of Guilin Medical University,Guilin 541001,China)

机构地区：[1]广西药用植物园西南濒危药材资源开发国家工程实验室,南宁530023 [2]广西医科大学第一附属医院感染性疾病科,南宁530023 [3]桂林医学院第一附属医院消化内科,桂林541001 [4]桂林医学院第一附属医院检验科,桂林541001

出　　处：《医药导报》2020年第9期1179-1184,共6页Herald of Medicine

基　　金：国家自然科学基金资助项目(81360659,81460127);广西科技合作与交流计划(桂科合14123001-18);广西壮族自治区卫生和计划生育委员会自筹经费科研课题(Z20180267)。

摘　　要：目的探讨荔枝核总黄酮(TFL)对四氯化碳(CCl 4)诱导肝纤维化大鼠和胆管结扎(BDL)肝纤维化大鼠的作用及其机制。方法大鼠40只,随机分正常对照组、CCl 4模型对照组、CCl 4+TFL组、BDL模型对照组、BDL+TFL组,每组8只。采用40%CCl 4诱导建立肝纤维化大鼠模型,双重胆管结扎法复制BDL模型。CCl 4+TFL组和BDL+TFL组灌胃给予TFL(300 mg·kg^-1·d^-1),正常对照组、CCl 4组和BDL组灌胃0.9%氯化钠溶液(300 mg·kg^-1·d^-1)。检测血清中碱性磷酸酶(ALP)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(T-BiL)。Sirius red染色测算胶原面积;免疫组织化学法检测抗α-平滑肌肌动蛋白(α-SMA)、CollagenⅠ在肝脏组织中的表达;Western blotting法检测α-SMA、核因子-κB(NF-κB)p65、环氧化酶2(COX-2)蛋白的表达。结果TFL降低CCl 4模型AST的作用强于BDL模型(P<0.05),而降低T-BiL、脾指数、肝脏α-SMA蛋白表达等的作用弱于BDL模型(P<0.05),均可明显降低两种模型大鼠ALT、ALP、肝纤维化的胶原面积、NF-κBp65与COX-2蛋白在肝脏中的表达(均P<0.05)。结论TFL对CCl 4和BDL两种模型的肝纤维化大鼠均有较好的改善肝纤维化作用,机制可能与调节炎症因子NF-κBp65、COX-2等的表达有关。Objective To compare the anti-fibrosis effect of total flavone of Litchi Chinensis Sonn.(TFL)on perchlormethane-induced and bild duct ligation(BDL)hepatic fibrosis model of rats.Methods Totally 40 male rats were randomly divided into five groups,namely normal control group,carbon tetrachloride(CCl 4)model control group,CCl 4+TFL group,BDL model control group,BDL+TFL group,with eight rats in each group.CCl 4 rats model was intragastric established by 40%CCl 4 twice a week.BDL rat model was established by double bile duct ligation.CCl 4+TFL group and BDL+TFL group were given TFL(300 mg·kg^-1·d^-1),normal control group,CCl 4 group and BDL group were given 0.9%sodium chloride solution(300 mg·kg^-1·d^-1).Serum alkaline phosphatase(ALP),alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(T-BiL)in the rats were detected.The liver tissues were stained by HE and Sirius red.Expressions ofα-smooth muscle actin(α-SMA)and CollagenⅠin liver tissues were assessed by immunohistochemical staining.And the expression ofα-SMA,nuclear factor-kappa B(NF-κB)p65,and cyclo-oxygenase 2(COX-2)in the liver tissues were determined by Western blotting.Results The effect of TFL in reducing the AST index in the CCl 4 model was better than that in the BDL model(P<0.05).The BDL model was superior in reducing the T-BiL,spleen index,andα-SMA protein expression(P<0.05).Both models significantly reduced the percent of liver collagen area,the serum of ALT,ALP,and the expression of NF-κBp65 and COX-2 protein in rat liver(P<0.05).Conclusion TFL has efficacy of anti-fibrosis,improving the liver function of the rats in CCl 4 and BDL liver fibrosis models.It is mainly anti-fibrotic and anti-inflammatory in CCl 4 model,and reduce jaundice and anti-inflammatory in BDL model.Its mechanism may be related with TFL inhibit expression of inflammatory factors NF-κB p65 and COX-2.

关 键 词：荔枝核总黄酮 肝纤维化 Α-平滑肌肌动蛋白 核因子-ΚBP65 环氧化酶2 

分 类 号：R975.5[医药卫生—药品] R965[医药卫生—药学]