白车轴草中总黄酮提取工艺研究  

Study on extraction process of total flavonoids from Trifolium repens

作　　者：王军捷 麦康明 温雪儿 任鹏[1] WANG Jun-jie;MAI Kang-ming;WEN Xue-er;REN Peng(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621000,Sichuan,China)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621000

出　　处：《湖北农业科学》2020年第13期123-126,共4页Hubei Agricultural Sciences

基　　金：四川省大学生创新创业训练计划资助项目(201810619061)。

摘　　要：基于超声波提取法探究提取白车轴草(Trifolium repens)中黄酮类物质的最佳工艺条件。以芦丁为空白对照,总黄酮得率为考察指标,通过亚硝酸钠-硝酸铝-氢氧化钠法,采用单因素试验及L9(33)正交试验设计,研究超声时间、乙醇浓度和液固比3种因素对白车轴草中黄酮类物质提取率的影响。结果表明,白车轴草总黄酮提取的最佳工艺条件为超声提取时间40 min、乙醇浓度50%、液固比60∶1(mL∶g)。在此条件下,白车轴草中总黄酮的提取率为2.853%。该试验设计方法高效、快速、经济,适用于白车轴草总黄酮提取工艺研究,并为白车轴草有效提取黄酮类物质提供试验依据。Based on the ultrasonic extraction method,the optimal technological conditions for extracting flavonoids from Trifolium re⁃pens was explored.With rutin as a blank control,the total flavonoid yield rate was an inspection index,and the effect of three factors,which were ultrasonic time,ethanol concentration and liquid-solid ratio,on the extraction rate of flavonoids in Trifolium repens was studied by the sodium nitrite-aluminum-sodium hydroxide method,single-factor test and L9(33)orthogonal test design.The results demonstrated that the optimal extraction process conditions of total flavonoids from Trifolium repens were ultrasonic time 40 min,etha⁃nol concentration 50%,liquid-solid ratio of 60∶1(mL∶g).Under these conditions,the extraction rate of total flavonoids in Trifolium repens was 2.853%.The test design method was highly efficient,fast and economical.It was suitable for the research on the extraction process of total flavonoids from Trifolium repens and provided experimental basis for the effective extraction of flavonoids from Trifoli⁃um repens.

关 键 词：白车轴草(Trifolium repens) 总黄酮 正交试验 提取工艺 

分 类 号：R284.2[医药卫生—中药学]