迟缓爱德华菌外膜蛋白PagC原核表达及免疫保护作用  

Prokaryotic Expression and Immunoprotection Effect of PagC Protein of Edwardsiella tarda

作　　者：张志强[1] 杨楠 李永慧 苏硕青 冯东青 吴同垒[1] 高桂生[1] 钱爱东[3] 史秋梅[1] ZHANG Zhi-qiang;YANG Nan;LI Yong-hui;SU Shuo-qing;FENG Dong-qing;WU Tong-lei;GAO Gui-sheng;QIAN Ai-dong;SHI Qiu-mei(Hebei Normal University of Science & Technology,Qinhuangdao,Hebei,066000,China;The Second Hospital of Qinhuangdao,Qinhuangdao,Hebei,066600,China;College of Animal Science and Technology,Jilin Agriculture University,Changchun,Jilin,130000,China)

机构地区：[1]河北科技师范学院,河北秦皇岛066004 [2]秦皇岛市第二医院,河北秦皇岛066604 [3]吉林农业大学动物科学技术学院,吉林长春130000

出　　处：《动物医学进展》2020年第9期1-4,共4页Progress In Veterinary Medicine

基　　金：中国博士后科学基金项目(2017M611935);河北省科技厅奖励性后补助项目(15926620H);秦皇岛市科技局科技支撑计划项目(201602A341);河北科技师范学院博士启动基金项目(2015YB002)。

摘　　要：为研究迟缓爱德华菌外膜蛋白PagC的免疫原性,用PCR方法扩增迟缓爱德华菌pagC基因,构建重组载体pET-32a-pagC,将其转化大肠埃希菌BL21后进行诱导表达,表达产物经SDS-PAGE和Western blot分析显示,重组蛋白大小约40 ku;将纯化的重组蛋白免疫小鼠后,以迟缓爱德华菌强毒株ET-13攻毒,结果该重组蛋白对免疫组小鼠具有95%的保护率。研究结果为重组PagC蛋白亚单位疫苗的研制奠定了基础。To investigate the immunogenicity of the outer membrane protein PagC from Edwardsiella tarda,the encoding PagC gene was amplified by PCR and cloned into pET-32a,a expression vector for recombinant PagC(rPagC)protein.The data of SDS-PAGE and Western blot analysis showed that,the rPagC could be detected and the molecular weight is about 40 ku.The data from mice model showed that the rPagC protein produced 95%protection rate against E.tarda strain ET-13 challenge at 48 days post vaccination.All these work provided foundation for development of subunit vaccine of E.tarda.

关 键 词：迟缓爱德华菌 外膜蛋白 PagC 原核表达 免疫保护 

分 类 号：S852.6[农业科学—基础兽医学]