弗格森埃希菌生长曲线及半数致死量测定  

Determination of Growth Curve and Half Lethal Dose of Escherichia fergusonii

作　　者：相思宇 陈建 闫可心 李明宣 闫宗斌 薛书江[1] XIANG Si-yu;CHEN Jian;YAN Ke-xin;LI Ming-xuan;YAN zong-bin;XUE Shu-jiang(Agricultural College of Yanbian University,Yanji,Jilin,133002,China;Animal Disease Prevention and Control Center of Yanbian Prefecture,Yanji,Jilin,133002,China)

机构地区：[1]延边大学农学院,吉林延吉133002 [2]延边州动物疫病预防控制中心,吉林延吉133000

出　　处：《动物医学进展》2020年第9期51-54,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31460657);吉林省科技厅青年科研基金项目(20150520129JH);吉林省教育厅“十三五”科学技术项目(JJKH20191134KJ)。

摘　　要：为测定弗格森埃希菌半数致死量,采用振荡比浊法(Dλ值法)测定弗格森埃希菌的生长曲线,并确定菌液与稀释倍数的关系,利用SPSS软件计算弗格森埃希菌对昆明小鼠的半数致死量(LD50)。结果显示,弗格森埃希菌在LB液体培养基中培养4 h^12 h时生长迅速,为对数生长期,在12 h以后进入稳定期;采用活菌计数法绘制弗格森埃希菌对数生长期OD值与活菌数的函数关系,得到回归方程y=11.09x+0.157,R^2=0.9955,具有良好的相关性;确定了弗格森埃希菌对昆明小鼠的半数致死量LD50为2.944×10^7 CFU,95%置信区间为1.557×10^7~4.817×10^7 CFU。In order to determine the growth curve and half lethal dose(LD50)of E.fergusonii,the test used the Oscillatory Turbidimetry(Dλvalue method)to measure the growth curve of E.fergusonii and determined the relationship between the bacterial solution and the dilutions.SPSS software was used to calculate the LD50 of E.fergusonii in Kunming mice.The results showed that E.fergusonii grows rapidly when cultured in LB liquid medium for 4 h-12 h.It is a logarithmic growth phase and enters a stable phase after 12 h.The OD value of E.fergusonii logarithmic growth phase is plotted using live bacteria counting method.As a function of the number of viable bacteria,the regression equation y=11.09x+0.157,R^2=0.9955,has a good correlation;it is determined that the LD50 of E.fergusonii to Kunming mice is 2.944×10^7 CFU.The 95%confidence interval is 1.557×10^7 to 4.817×10^7 CFU.

关 键 词：弗格森埃希菌 生长曲线 活菌数 半数致死量 

分 类 号：S852.61[农业科学—基础兽医学]