小麦TaNAC-B072基因的克隆和表达分析  

Cloning and Expression Analysis of TaNAC-B072 in Wheat

作　　者：陈文烨 杨帆[1] 刘永伟[1] 董福双[1] 赵和[1] 柴建芳[1] 吕孟雨[1] 周硕[1] CHEN Wen-ye;YANG Fan;LIU Yong-wei;DONG Fu-shuang;ZHAO He;CHAI Jian-fang;LV Meng-yu;ZHOU Shuo(Institute of Genetics and Physiology,Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei Province,Shijiazhuang 050051,China)

机构地区：[1]河北省农林科学院遗传生理研究所/河北省植物转基因中心,河北石家庄050051

出　　处：《江西农业学报》2020年第8期1-7,共7页Acta Agriculturae Jiangxi

基　　金：国家重点研发计划“七大作物育种”重点专项(2016YFD0101802);国家自然科学基金青年科学基金项目(31600216);河北省自然科学基金青年科学基金项目(C2017301066);河北省现代农业科技创新工程项目(2019-4-8-1)。

摘　　要：为了研究小麦NAC转录因子的功能及其对逆境胁迫的响应,本试验克隆了小麦的TaNAC-B072基因。该基因的开放阅读框长度为978 bp,编码蛋白长度为325个氨基酸,推测其分子量为35.75 kDa,等电点为7.06,属于中性蛋白,具有1个NAC结构域。多序列比对和系统进化树分析显示,TaNAC-B072与BdNAC72-like的亲缘关系较近。RT-PCR结果显示,在小麦的根和地上部分,TaNAC-B072基因的表达受高盐、渗透和冷胁迫的诱导,初步推测基因TaNAC-B072可能与小麦响应逆境胁迫有密切的关系。In order to study the function of wheat NAC gene and it response to stress,the TaNAC-B072 gene of wheat was cloned in the experiment.Sequence analysis showed that the open reading frame(ORF)of TaNAC-B072 was 978 bp,encoding 325 amino acids.Its protein contained a typical NAC domain,with molecular weight of 35.75 kDa and isoelectric point of 7.06.Multiple sequence alignment and phylogenetic tree analysis showed that TaNAC-B072 was closely related to the BdNAC72-like gene.The RT-PCR analysis showed that the expression of TaNAC-B072 was induced or inhibited by NaCl,osmotic and cold stress in root and shoot tissue,which suggested that the TaNAC-B072 gene might be closely related to plant resistance to abiotic stress.

关 键 词：小麦 NAC转录因子 基因克隆 表达分析 

分 类 号：S512.1[农业科学—作物学]