激光扫描共聚焦显微成像技术与应用  

Laser Scanning Confocal Microscopy and Its Application

作　　者：李成辉 田云飞[1] 闫曙光 LI Chenghui;TIAN Yunfei;YAN Shuguang(Analytical&Testing Center,Sichuan University,Chengdu 610064,China)

机构地区：[1]四川大学分析测试中心,成都610064

出　　处：《实验科学与技术》2020年第4期33-38,共6页Experiment Science and Technology

基　　金：国家自然科学基金(21705114);四川大学研究生教育教学改革研究项目(YJSJG027);四川大学基础研究培育专项(2019)。

摘　　要：激光扫描共聚焦显微镜已成为生物及医学等领域重要的研究手段。为了更充分、灵活地运用共聚焦显微镜的功能,提高仪器应用效果,拓展仪器使用需求,该文以尼康A1+共聚焦显微镜为例,介绍了共聚焦显微镜的基本原理、仪器配置和技术参数,样品制备要求,图像采集操作规程及技巧,日常维护和管理等方面信息。旨在为科研工作者获取高质量共聚焦图像提供有力的技术支持,提高工作效率,更好地服务于教学和科学研究。Laser scanning confocal microscopy has become crucial to biological and medical research.To fully and flexibly use the function,improve the application and performance,and boost the potential demand of confocal microscope,this article introduces the fundamental principle,instrument configuration and technical parameters,sample preparation requirements,image acquisition procedures and techniques,as well as daily maintenance and management information of Nikon A1+laser scanning confocal microscope.The aim is to provide a powerful technical reference for scientific researchers to obtain high-quality confocal images,improve work efficiency,and better assist teaching and scientific research.

关 键 词：激光共聚焦显微镜 原理 操作 荧光成像 

分 类 号：O657[理学—分析化学]