在本科微生物实验教学中拓展抑菌实验的探索  

Exploration of Comprehensive Bacteriostatic Experiment in Microbiology Experiment Teaching for Undergraduates

作　　者：熊莉 朱谦慧 汪红 牛霞 罗璇 陶瑜玮 李佛生 XIONG Li;ZHU Qianhui;WANG Hong;NIU Xia;LUO Xuan;TAO Yuwei;LI Fosheng(National Demonstration Center for Experimental Biology Education,Sichuan University,Chengdu 610064,China)

机构地区：[1]四川大学生物科学国家级实验教学示范中心,成都610064

出　　处：《实验科学与技术》2020年第4期88-92,共5页Experiment Science and Technology

基　　金：国家微生物资源平台(NIMR-2017-8X);四川省能源微生物资源共享平台(19PTDJ0029);基础学科拔尖学生培养计划(20180209)。

摘　　要：抑菌试验作为抗菌药物和抗生素替代物研发与应用中的关键技术,是生物类本科生需要熟练掌握的重要技能,然而目前国内大部分高校的基础微生物学实验课程中所开设的抑菌实验内容单一,教学设计传统,缺乏综合性。该研究新建了适合于本科教学的牛津杯法定量检测大蒜提取液抑菌效果的实验项目,并通过与原有定性实验项目有机整合,增加小组讨论和项目设计报告撰写环节,建立了一个“课上+课下”的综合性抑菌实验。拓展改进后的实验项目不仅能很好地激发学生的实验兴趣,更可以促进对学生综合实验技能和科研创新能力的培养。Bacteriostatic test,as a key technology in the R&D and application of antimicrobial agents and alternatives to antibiotics,is an important skill for undergraduates in biology program to be proficient in.However,the relevant experiments in the experiment courses for basic microbiology discipline of most Chinese universities are unvaried in content,outmoded in teaching design,and lack of comprehensiveness.In this study,a comprehensive bacteriostatic experiment featured by"on class+off class"teaching model is established,in which a new experiment of"quantitative detection of the antibacterial effect of garlic extract by Oxford cup method"is organically integrated with the initial qualitative experiment,and a group discussion and design report writing are also added.The improved model can not only stimulate students'interests in experiments,but also groom their comprehensive experimental skills and research innovation abilities.

关 键 词：微生物实验 抑菌试验 教学改革 综合性 

分 类 号：R33[医药卫生—人体生理学]