慢性乙型肝炎患者外周血单个核细胞Tim-3和血清IL-33表达及临床意义  

Clinical significance of expression of Tim-3 on peripheral blood mononuclear cells and serum IL-33 in diagnosis of patients with chronic hepatitis B

作　　者：刘琼[1] 杨歆[1] 唐简[1] 彭忠田[1] 蒋孝华[1] LIU Qiong;YANG Xin;TANG Jian;PENG Zhong-tian;JIANG Xiao-hua(The First Affiliated Hospital of University of South China,Hengyang,Hunan 421001,China)

机构地区：[1]南华大学附属第一医院感染科,湖南衡阳421001

出　　处：《中华医院感染学杂志》2020年第13期2014-2017,共4页Chinese Journal of Nosocomiology

基　　金：湖南省卫生健康委科研计划资助项目(C2019118)。

摘　　要：目的探究外周血单个核细胞(PBMCs)T细胞免疫球蛋白黏蛋白分子-3(Tim-3)和血清白细胞介素-33(IL-33)的表达水平对检测慢性乙型肝炎(CHB)患者病情诊断意义。方法选取南华大学附属第一医院收治的60例CHB患者为病例组,并选取同期进行体检的62名健康人为对照组。采用流式细胞仪检测外周血PBMCs表面Tim-3表达水平;采用酶联免疫吸附法(ELISA)检测血清IL-33水平;分别采用ELISA法、赖氏法、溴甲酚绿法检测两组患者AST、ALT和ALB水平;采用Spearman秩相关分析法分别对两组外周血PBMCs表面Tim-3和血清IL-33的表达水平、病例组PBMCs表面Tim-3和血清IL-33的表达水平与血清ALT、AST、ALB水平进行相关性分析。结果病例组PBMCs表面Tim-3和血清IL-33的表达水平均高于对照组(P<0.05);病例组血清ALT、AST水平均高于对照组,血清ALB水平低于对照组(P<0.05);病例组PBMCs表面Tim-3和血清IL-33表达水平和血清ALT、AST水平呈正相关,与血清ALB水平呈负相关(P<0.05);对照组和病例组PBMCs表面Tim-3和血清IL-33的表达水平均呈负相关(P<0.05)。结论Tim-3和IL-33表达水平越高,CHB患者肝脏损伤越重,两者联合检测对其诊断具有较高的临床价值。OBJECTIVE To explore the clinical significance of expression of T cell immunoglobulin mucin molecule-3(Tim-3)on peripheral blood mononuclear cell(PBMCs)and serum interleuin-33(IL-33)in diagnosis of illness condition of the patients with chronic hepatitis B(CHB).METHODS Totally 60 patients with CHB who were treated in the First Affiliated Hospital of University of South China were chosen as the case group,meanwhile,62 healthy people who received physical examination were set as the control group.The expression level of Tim-3 on PBMCs was detected by using flow cytometry,the serum IL-33 level was detected by means of enzyme-lined immunosorbent assay(ELISA),the levels of AST,ALT and ALB of the two groups of patients were detected by respectively using ELISA,Reisler’s method and bromocresol green method;Spearman rank correlation analysis was performed for the correlation between the Tim-3 on surface of PBMCs and the serum IL-33 level of the two groups,the Tim-3 on PBMCs and serum IL-33 level of the case group and the levels of serum ALT,AST and ALB.RESULTS The levels of Tim-3 on surface of PBMCs and serum IL-33 of the case group were significantly higher than those of the control group(P<0.05);the levels of serum ALT and AST of the case group were significantly higher than those of the control group,while the serum ALB level of the case group was significantly lower than that of the control group(P<0.05).The levels of Tim-3 on surface of PBMCs and serum IL-33 of the case group were positively correlated with the levels of serum ALT and AST but were negatively correlated with the serum ALB level(P<0.05).The Tim-3 on surface of PBMCs of both the case group and the control group was negatively correlated with the serum IL-33 level(P<0.05).CONCLUSION The higher the levels of Tim-3 and IL-33,the more severe the liver damage of the patients with CHB is.The combined detection of Tim-3 and IL-33 has high clinical value in diagnosis of the liver damage of the CHB patients.

关 键 词：慢性乙型肝炎 T细胞免疫球蛋白黏蛋白分子3 白细胞介素-33 外周血单个核细胞 

分 类 号：R512.62[医药卫生—内科学]