Facile thermal decomposition synthesis of sub-5 nm nanodots with long-lived luminescence for autofluorescence-free bioimaging  

高温热分解法制备亚5 nm的长余辉纳米点用于无背景生物成像

作　　者：Xiaobo Lv Na Chen Jie Wang Quan Yuan 吕小波;陈娜;王杰;袁荃(Key Laboratory of Analytical Chemistry for Biology and Medicine(Ministry of Education),College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072,China;Institute of Chemical Biology and Nanomedicine,State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China)

机构地区：[1]Key Laboratory of Analytical Chemistry for Biology and Medicine(Ministry of Education),College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072,China [2]Institute of Chemical Biology and Nanomedicine,State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China

出　　处：《Science China Materials》2020年第9期1808-1817,共10页中国科学（材料科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(21925401 and 21904100)。

摘　　要：Persistent nanophosphors can remain luminescent after the removal of the excitation.Persistent nanophosphors exhibit great advantages in biomedical fields,particularly in autofluorescence-free bioimaging and in-siteexcitation-free photo-theranostics.Despite the great promise of persistent nanophosphors in biomedicine,studies on the controlled synthesis of persistent nanophosphors are limited.Herein,a metal acetylacetonate-based thermal decomposition method was developed for the synthesis of ultra-small persistent luminescence nanodots(PLNDs).The PLNDs display uniform size,good dispersibility and strong persistent luminescence.The luminescent properties of the PLNDs can be readily regulated by ion doping.The thermal decomposition method shows excellent versatility in the synthesis of PLNDs including gallate,sulfide and fluoride.Due to their ultra-small size and surface adsorbed hydrophobic ligand,the PLNDs can be easily integrated with liposomes to construct a stable and biocompatible persistent luminescent nanoplatform for biomedical applications.This work puts forwards a versatile method for the controlled synthesis of ultra-small persistent nanophosphors,and it may further contributes to the areas ranging from biosensing to cancer therapy.长余辉是指激发光关闭后发光仍然持续的现象.长余辉纳米材料在生物医学领域,尤其是无背景生物成像和光学治疗方面,表现出巨大的优势.虽然长余辉材料具有广阔的生物医学应用前景,但是长余辉纳米材料的控制合成研究非常少.目前,长余辉纳米材料基本都是通过煅烧后研磨等"自上而下"的方法制备的,得到的纳米颗粒分散性差、尺寸不均一、表面难以修饰.发展长余辉纳米材料的控制合成方法对推进长余辉材料的生物医学应用具有重要价值.在本文中,我们提出一种基于乙酰丙酮金属盐的高温热分解方法用于合成超小尺寸的长余辉纳米点.合成的长余辉纳米点粒径均一,尺寸在5 nm左右,具有良好的分散性和明亮的长余辉发光.通过离子掺杂可以有效地调控长余辉的发光性能.该方法适用于镓酸盐、硫化物、氟化物等长余辉纳米点的控制合成,具有良好的普适性.基于长余辉纳米点的超小尺寸和表面吸附的疏水性配体,我们构建了脂质体@长余辉纳米点复合生物成像平台,该平台结合了长余辉纳米点的长寿命发光特点和脂质体的生物相容性好、负载量高等特点,在生物成像、药物递送等领域有潜在应用价值.我们的工作为长余辉纳米材料控制合成提供了新思路,对长余辉纳米材料的生物医学应用具有潜在的推动作用.

关 键 词：persistent luminescence thermal decomposition nanoparticles BIOIMAGING 

分 类 号：TB383.1[一般工业技术—材料科学与工程]