双酚S对高脂饮食斑马鱼脂代谢的影响及机制  

Effects and mechanism of BPS on lipid metabolism of zebrafish with high-fat diet

作　　者：余翠 武琳 陈忠[2,3] 杨明[4] YU Cui;WU Lin;CHEN Zhong;YANG Ming(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306;Department of Cardiology,Shanghai University of Medicine and Health Sciences Affiliated Sixth People’s Hospital East,Shanghai 201306;Department of Cardiology,Shanghai Jiao Tong University Affiliated Sixth People’s Hospital East,Shanghai201306;College of Environmental and Chemical Engineering,Shanghai University,Shanghai 200444,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]上海健康医学院附属第六人民医院东院心内科,上海201306 [3]上海交通大学附属第六人民医院东院心内科,上海201306 [4]上海大学环境与化工学院,上海200444

出　　处：《南京医科大学学报（自然科学版）》2020年第7期981-985,1020,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：上海健康医学院师资人才百人库项目(2017026);上海市第六人民医院东院项目(院学科2017006)。

摘　　要：目的:研究双酚S(bisphenol S,BPS)对斑马鱼脂代谢的影响及可能机制。方法:用高脂饮食饲养的斑马鱼幼鱼为模型,研究不同浓度BPS暴露对斑马鱼尾部血管中脂质沉积的影响,进一步通过ELISA和荧光定量RT-PCR检测脂代谢相关蛋白和基因的表达水平来研究其可能机制。结果:在1和10μg/L的BPS暴露下,幼鱼血管中胆固醇积累显著降低(P<0.01)。在饲料为42 mg/皿投喂量下,BPS暴露组斑马鱼血管中脂质积累量均显著性下降(P<0.01)。100μg/L的BPS暴露组中RXRα蛋白表达量显著性降低(P<0.05);10μg/L BPS暴露可显著促进hmgcrα基因的表达(P<0.05),而ldlrα和rxrα的表达分别在100和1000μg/L BPS暴露下受到显著抑制(P均<0.05),rorα基因表达量在0.1、1.0、100.0μg/L BPS暴露后显著降低(P均<0.05),1000μg/L的BPS暴露后降低更加明显(P<0.01)。结论:BPS对斑马鱼幼鱼脂代谢的影响与高脂饲料的投喂量及脂代谢相关基因的表达异常有关,其影响机制可能与rxrα/ror信号通路相关。Objective:To study the effects of bisphenol S(BPS)on lipid metabolism in zebrafish with high-fat diet and its potential mechanism.Methods:The effects of BPS in different concentration on lipid deposition in the blood vessels of zebrafish with high-fat diet were studied,and the changes of lipid metabolism-related proteins and genes were detected by ELISA and quantitative RT-PCR.Results:The cholesterol accumulation in the larvae blood vessels was significantly decreased under the exposure of 1 and 10μg/L BPS(P<0.01).When fed with high-fat diet 42 mg/dish,the lipid accumulation in the larvae blood vessels of the BPS exposed group was also significantly decreased(P<0.01).The expression level of RXRαprotein was significantly reduced in the 100μg/L BPS exposed group(P<0.05).The gene expression of hmgcrαwas significantly induced by 10μg/L BPS exposure(P<0.05),and the m RNA levels of ldlrαand rxrαwere all significantly decreased in the 100μg/L and 1000μg/L BPS exposed group(all P<0.05).While the expression of rorαgene was reduced after exposure of 0.1,1.0,100.0μg/L BPS(all P<0.05),and it was lower after exposure of 1000μg/L BPS(P<0.01).Conclusion:The effect of BPS on lipid metabolism of zebrafish larvae was related to the feeding amount of high-fat diet and abnormal expression of lipid metabolism related genes,and its mechanism may be related to rxr/ror signaling pathway.

关 键 词：BPS 斑马鱼 脂代谢 信号通路 

分 类 号：R589.2[医药卫生—内分泌]