甘肃省半夏立枯病病原鉴定  

Identification of the pathogen of Pinellia ternate damping off in Gansu,China

作　　者：魏莉霞[1] 胡永建 卢晓红 高微微[4] 陈文庆[1] WEI Lixia;HU Yongjian;LU Xiaohong;GAO Weiwei;CHEN Wenqing(Institute of Economic crops and Beer Materials,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China;Institute of Quality Standards and Testing Technology,Henan Academy of Agricultural Sciences,Zhengzhou Henan 450002,China;Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China)

机构地区：[1]甘肃省农业科学院中药材研究所,甘肃兰州730070 [2]河南省农业科学院农业质量标准与检测技术研究所,河南郑州450002 [3]中国农业科学院植物保护研究所,北京100193 [4]中国医学科学院中国协和医科大学药用植物研究所,北京100193

出　　处：《中兽医医药杂志》2020年第4期75-77,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省农业科学院科技支撑计划(2016GAAS05);甘肃省农业科学院创新团队项目(2017GAAS29);甘科协学20190002。

摘　　要：对甘肃省陇南市西和县半夏种植区发生半夏立枯病大田发病特点进行详细观察,经病原菌分离培养、柯赫氏法则证病、病原菌形态学和分子生物学鉴定确定该病的病原。结果发现,半夏田间发病明显症状为植株青枯,叶片蜷曲、渐渐枯萎,茎基部溢缩、干枯。菌落在PDA平板上25℃培养生长速度快,菌丝有隔膜,初期无色,老熟时浅褐色至黄褐色,分枝处成直角,菌落棕色,生长均匀,后期平板上会出现棕褐色团状菌丝,菌落背面土黄色至黄褐色。柯赫氏法则证病,明确其病原为立枯丝核菌Rhizoctonia solani。In recent years,symptoms of damping off were observed on P.ternate in Xihe county,Longnan city,Gansu province. Through the pathogen isolation and culture, the disease was confirmed by Koch’s rule, and based on the morphological and molecular identification,the pathogen was identified as Rhizoctonia solani. The obvious symptoms were plant withered,leaves curled,stem base constriction and dry. The growth rate of the colony was fast when cultured at 25 ℃ on PDA plate. The mycelium had septum,colorless at the early stage,light brown to yellowish brown when it was old and ripe,branches were at right angles,the colony was brown. At last,brown clumps of mycelium appeared on the plate,and the backside of the colony was earthen to yellowish brown.

关 键 词：半夏 立枯病 立枯丝核菌Rhizoctonia solani 鉴定 茎基部 

分 类 号：S853.72[农业科学—临床兽医学]