过表达毛白杨线粒体APX基因烟草提高抗逆能力的研究  

Tobacco overexpression Populus tomentosa mitochondria ascorbate peroxidase improving stress resistance

作　　者：王兵 程子义 张蕾 赵芝婧 陆海 刘頔 Wang Bing;Cheng Ziyi;Zhang Lei;Zhao Zhijing;Lu Hai;Liu Di(National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration,School of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学生物科学与技术学院,林木育种国家工程实验室,林木、花卉遗传育种教育部重点实验室,国家林业局树木花卉育种与生物工程重点开放实验室,北京100083

出　　处：《北京林业大学学报》2020年第7期33-39,共7页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(31971618)。

摘　　要：【目的】为了研究毛白杨线粒体APX(PtomtAPX)在抗逆过程中的作用,本研究对过表达PtomtAPX的转基因烟草进行抗逆研究。【方法】对过表达PtomtAPX烟草和野生型烟草进行干旱、盐、氧化胁迫处理后,测量相对含水量、叶绿素含量、丙二醛含量、APX活性、AsA消耗量、NADP/NADPH比值和SOD活性。【结果】通过对比转基因烟草植株与野生型植株的生长差异发现,在氧化胁迫、盐胁迫和干旱胁迫下,转PtomtAPX基因烟草的APX活性、相对含水量、叶绿素含量、AsA消耗量、NADP/NADPH比值升高量均明显高于野生型,其中转PtomtAPX基因烟草的APX活性为野生型的1.77倍,平均相对含水量为野生型的1.15倍,叶绿素含量为野生型的1.6倍,AsA消耗量为野生型的1.11倍,NADP/NADPH值为野生型的1.18倍,表明过表达PtomtAPX转基因植株清除活性氧的能力更强。【结论】在非生物胁迫下,PtomtAPX能消除H2O2,防止细胞损伤,在植物抗逆中发挥重要作用。[Objective]In order to study the role of Populus tomentosa mitochondrial APX(PtomtAPX),the stress resistance of overexpression PtomtAPX transgenic tobacco was studied in this paper.[Method]Overexpression PtomtAPX transgenic tobacco and wild type tobacco were treated with drought stress,salt stress and oxidative stress.Relative water content,chlorophyll content,malondialdehyde content,APX activity,AsA consumption,NADP/NADPH ratio,SOD activity were measured.[Result]In this study,compared wild type plants with overexpression PtomtAPX,under oxidative stress,salt stress and drought stress,the APX activity,relative water content,chlorophyll content,AsA consumption,and NADP/NADPH ratio increase of the tobacco overexpression PtomtAPX gene were significantly higher than those of wild type,of which the APX activity of PtomtAPX transgenic tobacco was 1.77 times of wild type,the average relative water content was 1.15 times of wild type,the chlorophyll content was 1.6 times of wild type,AsA consumption was 1.11 times of wild type,and the ratio of NADP to NADPH was 1.18 times of the wild type.It suggested that the tobacco overexpressed PtomtAPX harbored stronger ROS scavenging ability.[Conclusion]These indicate that mitochondrial APX acts a crucial role of abiotic stress tolerance in plants by eliminating H2 O2 and preventing cell damage under abiotic stress.

关 键 词：抗坏血酸过氧化物酶 转基因烟草 非生物性胁迫 毛白杨 

分 类 号：S718.43[农业科学—林学] S792.117