新疆巴音布鲁克地区森林革蜱钙网蛋白基因部分片段克隆与序列分析  

Cloning and sequence analysis of partial calreticulin gene sequence of Dermacentor silvarum in Bayinbuluk area,Xinjiang,China

作　　者：胡政香 呼尔查 刘丹 赵明明 吉尔格力[1] 乔格拉森[1] Hu Zhengxiang;Hu Ercha;Liu Dan;Zhao Mingming;Ji Ergeli;Qiao Gelasen(School of Biological Engineering,Bayingol Vocational and Technical College,Xinjiang Korla 841000;College of Animal Science,Xinjiang Agricultural University,Xinjiang Urumqi 830000)

机构地区：[1]巴音郭楞职业技术学院生物工程学院,新疆库尔勒841000 [2]新疆农业大学动物科学学院,新疆乌鲁木齐830000

出　　处：《现代畜牧兽医》2020年第7期14-19,共6页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：巴音郭楞职业技术学院2019年度院级科研课题(bykj 2019n-3)。

摘　　要：为了进一步研究森林革蜱钙网蛋白(DsCRT)功能,试验应用PCR方法和生物信息学分析软件预测森林革蜱钙网蛋白基因的结构和功能。通过GenBank比对DsCRT基因和氨基酸序列;利用生物信息学软件对序列进行预测和分析。结果表明:PCR扩增的DsCRT序列长度为933 bp,截短序列编码311个氨基酸,蛋白质分子质量约为35.88 ku,理论等电点约为4.74,亲水性较高;Ds CRT具有良好的抗原表位。系统进化树显示DsCRT基因与变异革蜱和安氏革蜱的亲缘关系最近。说明该蛋白具有较高的免疫原性,且与同属其他种的钙网蛋白具有较高同源性测序获得序列信息为进一步分析DsCRT蛋白的功能提供参考。To further enhance knowledge about calreticulin of D.silvarum,PCR and bioinformatics analysis were used to predict the structure and function of putative D.silvarum calreticulin(DsCRT).The results showed that the length of the truncated DsCRT sequence amplified by PCR was 933 bp,encoding 311 amino acids.The molecular weight of the predicted protein was about 35.88 ku.The theoretical isoelectric point was about 4.74,and the hydrophilicity was high.The DsCRT protein has good antigenicity by prediction.The phylogenetic tree showed that the DsCRT gene was closely related to that of D.variabilis and D.occidentalism,while a little distant from Hyaloma and Rhipicephalus.The sequence information obtained in the study provided a reference for further analysis on the function of D.silvarum calreticulin.

关 键 词：森林革蜱 钙网蛋白 序列分析 

分 类 号：S855.9[农业科学—临床兽医学]