Investigation of Salt Stress Response Mechanisms Using Mirna Sequencing of Salt Tolerant and Salt Sensitive Jute Genotypes  

miRNA序列分析耐盐型和敏感型黄麻基因型在盐胁迫下的应答机制

作　　者：WU Yu-peng YANG Ze-mao SU Jian-guang LIU Wen-xiang 吴宇鹏;杨泽茂;粟建光;刘文祥(中国农业科学院麻类研究所,湖南长沙410205;湖南农业大学生物科学技术学院,湖南长沙410205;湖南省农业信息与工程研究所,湖南省农业科学院,湖南长沙410125)

机构地区：[1]Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410205,PRC [2]College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410205,PRC [3]Hunan Institute of Agricultural Information and Engineering,Hunan Academy of Agricultural Sciences,Changsha 410125,PRC

出　　处：《Agricultural Science & Technology》2020年第2期32-40,共9页农业科学与技术（英文版）

基　　金：湖南省自然科学基金(2017JJ3350);国家自然科学基金(31601351)。

摘　　要：Salt is a major environmental stressor for crops.MicroRNAs(miRNAs)play a crucial role in plant response to salt stress.In this study,a comparative analysis of miRNAs between salt-tolerant and salt-sensitive jute subjected to high salinity were carried out.374 known mature miRNAs were identified and 87 potential miRNA candidates from the eight sRNA libraries were predicted.For the salt-stressed versus the control,181 miRNAs including 45 novel miRNAs were differentially expressed.More differentially expressed miRNAs were present in TC(salt tolerant)than in NY(salt sensitive).Among NY,58 and 27 differentially expressed miRNAs were discovered in leaves and roots,respectively,whereas among TC,45 and 113 differentially expressed miRNAs were discovered in leaves and roots,respectively.Two common differentially expressed miRNAs were discovered in both leaves and roots of the two genotypes.10 differentially expressed miRNAs were common across both leaves and roots only in TC,including 6 upregulated,3 downregulated and one both up and down-regulated miRNA.The results obtained in this analysis contribute to an improved understanding of abiotic-stress tolerance in jute and will be useful for breeding programs to improve jute productivity in soils with high salinity.盐胁迫是农作物生长的主要环境胁迫源之一,其中MicroRNAs(miRNAs)的表达在植物对盐胁迫响应中起决定性作用。该研究对盐耐受型(TC)和盐敏感型(NY)黄麻在高盐胁迫下miRNAs的表达进行比较分析。从8个sRNA文库中鉴定出了374个预期的miRNAs,并预测了87个潜在的候选miRNAs。在盐胁迫和对照之间,包括45个特异的miRNAs在内的181个miRNAs有差异表达,且TC中差异表达的miRNAs较NY多。在NY的叶片和根中分别发现58和27个miRNAs差异表达,而在TC的叶片和根中分别发现45和113个miRNAs的差异表达。在两种基因型组织中发现存在两个共同的差异表达miRNA。仅在TC中,发现10个共通的miRNAs在叶片和根组织中有差异表达,包括6个上调、3个下调和1个上调和下调的miRNA。该研究将有助于提高大家对黄麻非生物胁迫耐性的认识,并有助于提高黄麻在高盐碱地的产量。

关 键 词：JUTE miRNA Salt stress Salt-sensitive SALT-TOLERANT 

分 类 号：S563.4[农业科学—作物学]