云南省珠芽魔芋(Amorphophallus bulbifer)软腐病病原菌鉴定  

Identification of a soft rot disease pathogen of Amorphophallus bulbifer in Yunnan Province

作　　者：魏环宇[1,2] 魏薇 杨敏 裴卫华 赵建荣[2] 钟宇[2] 刘佳妮[2] 苏源 黄飞燕[2] 王启宇[2] 胡文婷 陈海如 余磊 WEI Huan-yu;WEI Wei;YANG Min;PEI Wei-hua;ZHAO Jian-rong;ZHONG Yu;LIU Jia-ni;SU Yuan;HUANG Fei-yan;WANG Qi-yu;HU Wen-ting;CHEN Hai-ru;YU Lei(College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;College of Agronomy/Yunnan Urban Agricultural Engineering and Technological Research Center,Kunming University,Kunming 650214,China;Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming 650223,China)

机构地区：[1]云南农业大学植物保护学院,昆明650201 [2]昆明学院农学院/云南省都市特色农业工程技术研究中心,昆明650214 [3]云南省农业科学院生物技术与种质资源研究所,昆明650223

出　　处：《植物病理学报》2020年第4期381-386,共6页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(31660426);云南省地方本科高校基础研究联合专项(2017FH001-005);云南省科技厅人才专项(2018HB100);云南省教育厅科学研究基金项目(2018JS389)。

摘　　要：魔芋(Amorphophallus spp.)是我国西南地区广泛种植的一种重要经济作物。2016~2018年在云南省发现一种由细菌侵染引起的珠芽魔芋新病害,症状表现为茎秆或叶片出现水渍状病斑,茎秆软化下垂,根部及球茎腐烂变褐,甚至整株倒伏。根据病害发病症状,并通过菌落形状、致病性测定、Biolog和16S rDNA测序分析,结果显示该病原菌在LB培养基上菌落呈近圆形,中央隆起,淡奶油色,质地均匀,表面光滑发亮;扫描和透射电子显微镜下观察显示,菌体形态呈短杆状,周生鞭毛,两端稍钝圆;基于16S rDNA扩增序列系统进化分析与已报道的Pectobacterium aroidearum strain CL1904-3(MK875007)相似性达99%以上,构建的系统进化树表明与P.aroidearum菌株亲缘关系最近,Biolog测定和16S rDNA序列分析均将该病原菌鉴定为软腐果胶杆菌(P.aroidearum)。这是我国首次报道由P.aroidearum引起的珠芽魔芋软腐病害。研究结果进一步加深了人们对该病害的认识,为其防治提供了科学依据。Amorphophallus spp.is widely planted in southwest China as an important cash crop.A new disease of konjac caused by bacterial infection was found in Yunnan province from 2016 to 2018.Symptoms include watermarks on the stem or leaves,softening and sagging of the stem,browning of the roots and bulbs,and eventually lodging of the entire plant.Identification and analysis were carried out by observation of disease symptoms and colony morphology,pathogenicity determination,Biolog test and 16S rDNA sequencing.The results showed that the colonies of this pathogen were nearly round in LB medium,with central bulge,light cream color,uniform texture and smooth and shiny surface.Observation under scanning and transmission electron microscope showed that the morphology of thallus was short rod-shaped with periflagellum and slightly obtuse circle at both ends.Systematic evolution analysis based on 16S rDNA amplification sequence has more than 99%similarity with the reported Pectobacterium aroidearum strain CL1904-3(MK875007).The phylogenetic tree constructed showed the closest relationship with P.aroidearum.Biolog test and 16S rDNA sequence analysis identified the pathogen as P.aroidearum.This is the first report of soft rot of P.aroidearum in China.The identification of new pathogen of soft rot of konjac deepens the understanding of this disease and provides scientific basis for the control of this disease.

关 键 词：珠芽魔芋 软腐病 病原鉴定 

分 类 号：S432.42[农业科学—植物病理学]