本氏烟柯浩体蛋白基因全长克隆及其功能预测  

The full-length cloning and functional prediction of Nicotiana benthamiana cajal body protein

作　　者：郭小囡 李阳 谢理 郑璐平[1] GUO Xiao-nan;LI Yang;XIE Li;ZHENG Lu-ping(Key Laboratory of Biopesticide and Chemical Biology,Ministry of Education,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室,福州350002

出　　处：《植物病理学报》2020年第4期420-425,共6页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助(31871926);福建省自然科学基金资助(2019J01654)。

摘　　要：柯浩体蛋白coilin是细胞器柯浩体的重要指示蛋白,参与病毒侵染。本文根据拟南芥柯浩体蛋白(Atcoilin)在本氏烟基因组数据库中的比对结果,确定其对应的靶基因并设计引物,首次获得本氏烟柯浩体蛋白(Nbcoilin)基因全长序列3017 bp,编码区为2409 bp,编码803个氨基酸;具有柯浩体蛋白3个典型功能域以及一个类似动物柯浩体蛋白特有的精氨酸/甘氨酸富集区("RGG"box);系统发育树显示该蛋白归属植物coilin分支,亚细胞定位显示该蛋白定位在柯浩体上,确定该蛋白是coilin的同源蛋白。研究结果为探究Nbcoilin的功能及其与病毒互作奠定了基础。Coilin is a marker of cajal body(CB),which involves in virus infection.Based on the blast results of Arabidopsis thaliana coilin against Nicotiana benthamiana genome database,primers were designed to amplify the putative full-length Nbcoilin gene with 3017 bp in length.The complete coding region for this gene is 2409 bp in length,encoding 803 amino acids.It contains the typical functional domains of coilin protein and an analog motif of the animal coilin‘RGG'box.Phylogenetic analysis showed that the cloned Nbcoilin gene was placed in a cluster with other plant coilins.The subcellular localization analysis showed Nbcoilin was localized to the cajal bodies.These results indicated that the cloned Nbcoilin gene is a homologous protein of coilin,and laid a foundation for further studying the function of Nbcoilin and its interaction with plant viruses.

关 键 词：柯浩体 COILIN 功能域预测 亚细胞定位 

分 类 号：S432.1[农业科学—植物病理学]