宁夏设施番茄病毒病病原鉴定  

Identification of the viral pathogens infecting tomato in Ningxia

作　　者：邓杰 王炜哲 杨璐嘉 马占鸿[1] DENG Jie;WANG Wei-zhe;YANG Lu-jia;MA Zhan-hong(Department of Plant Pathology,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学植物病理学系,北京100193

出　　处：《植物病理学报》2020年第4期494-498,共5页Acta Phytopathologica Sinica

基　　金：宁夏回族自治区重点研发计划重大项目(2016BZ09、2017BY080)。

摘　　要：番茄病毒病是番茄(Solanum lycopersicum)安全生产的主要限制因素,感染番茄作物的病毒种类高达136种[1]。小RNA测序和组装技术(small RNA sequencing and assembly,sRSA)已用于不同物种的病毒检测[2]。Xu等[2]对采自我国的170个番茄样本进行小RNA深度测序分析,鉴定出22种病毒。其采样地点未包括宁夏回族自治区。Tomato,being the main cultivated vegetable in Ningxia,is sensitive to virus disease which has caused great economic losses to local farmers.In order to identify the main pathogen of tomato virus disease in Ningxia and take pertinently appropriate measures for disease prevention,we collected 72 tomato plant samples with suspected symptoms of virus disease during May 2018 in the main tomato-producing areas of Ningxia.A total of 4 viruses were identified by small RNA sequencing and assembly technology,including tomato spotted wilt virus(TSWV),tomato yellow leaf curl virus(TYLCV),southern tomato virus(STV)and tomato chlorotic virus(ToCV).The coverage of the spliced virus sequences in the viral gene reference sequences was up to 81.3%-100%,and the homology was 93.59%-99.77%.The identification accuracy of the four viruses were verified again by PCR/RT-PCR,Sanger sequencing and comparison.STV and ToCV were identified in Ningxia for the first time.This study offers insights into census of plant virus disease and provides valuable information to facilitate the management of viral diseases.

关 键 词：番茄病毒病 限制因素 病原鉴定 采样地点 病毒检测 组装技术 病毒种类 深度测序 

分 类 号：S432.41[农业科学—植物病理学]