甘薯病毒2中国分离物全基因组序列克隆及遗传进化分析  

Complete nucleotide sequence and phylogenetic analysis of the sweet potato virus 2 isolated from China

作　　者：秦艳红[1,2,3] 乔奇 王爽[1,2,3] 张德胜 王永江[1,2,3] 田雨婷 张振臣[1,2,3] QIN Yan-hong;QIAO Qi;WANG Shuang;ZHANG De-sheng;WANG Yong-jiang;TIAN Yu-ting;ZHANG Zhen-chen(Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Henan Key Laboratory of Crop Pest Control,Zhengzhou 450002,China;IPM Key Laboratory in Southern part of North China for Ministry of Agriculture,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院植物保护研究所,郑州450002 [2]河南省农作物病虫害防治重点实验室,郑州450002 [3]农业部华北南部作物有害生物综合治理重点实验室,郑州450002

出　　处：《植物病理学报》2020年第4期505-508,共4页Acta Phytopathologica Sinica

基　　金：国家甘薯产业技术体系建设项目(CARS-10-B13);河南省农业科学院自主创新基金项目(2019ZC37);河南省科技攻关项目(182102110274)。

摘　　要：甘薯病毒2(Sweet potato virus 2,SPV2)是马铃薯Y病毒科(Potyviridae)马铃薯Y病毒属(Potyvirus)成员。SPV2也称为甘薯脉花叶病毒(ipomoea vein mosaic virus,IVMV)和甘薯Y病毒(sweet potato virus Y,SPVY)[1],是甘薯上常见的病毒之一。SPV2病毒粒体为线条状,长度为850 nm,在细胞质中形成风轮状或卷轴状内含体[2]。The complete nucleotide sequence of a Chinese isolate of sweet potato virus 2(SPV2-Ch1)was determined by using RT-PCR and rapid amplification of cDNA ends methods.Sequence comparisons of complete genomic sequence,polyprotein,and individual protein sequences were performed using DNAMAN.Phylogenetic tree of SPV2-Ch1 isolate with other SPV2 isolates were generated using MEGA7.0 software.Sequence analysis revealed that the complete genome of SPV2-Ch1 was 10747 nucleotides(nt)including a poly(A)tail at the 3'-end.The 5'-untranslated region(5'-UTR)and 3'-UTR is 107 nt and 239 nt in length,respectively.The genome of SPV2-Ch1 contained an open reading frame of 10401 nt encoding for a putative polyprotein of 3466 amino acids.Pairwise comparisons showed that SPV2-Ch1 and other SPV2 isolates available in GenBank database shared 80.3%-98.7%identity at the complete genome nucleotide sequence level.SPV2-Ch1 has the highest nt identity with the isolate SC6(98.7%)and lowest nt similarity with isolate SSBles-74_ZA(80.3%).Phylogene-tic tree analysis based on polyprotein gene indicated that isolates SPV2-Ch1,AM-MB2,AuScan,CW142,GJ118,GWB-2,HN77,LSU2,SC6,SCN20 and TM37 formed one group.This is the first report of the complete genomic sequence of SPV2 isolated from China.

关 键 词：马铃薯Y病毒 病毒粒体 全基因组序列 花叶病毒 甘薯病毒 遗传进化分析 线条状 

分 类 号：S432.41[农业科学—植物病理学]